Anti-Acetylcholinesterase antibody [AE-2] (ab77959)

Overview

• Product nameAnti-Acetylcholinesterase antibody [AE-2]See all Acetylcholinesterase primary antibodies ...
• Description
  Mouse monoclonal [AE-2] to Acetylcholinesterase
• Tested applicationsFlow Cyt, WB, IP, IHC-FoFr more details
• Species reactivity
  Reacts with: Rat, Human, Monkey
  
• Immunogen

  Full length native Acetylcholinesterase protein (purified from Human erythrocyte)

• Positive controlSKBR-3 cell lysate.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.1% Sodium Azide
  Constituents: Ascites, 0.25M Sodium chloride, 0.02M PBS, pH 7.2
• Concentration information loading...
• PurityAscites
• Clonality Monoclonal
• Clone numberAE-2
• IsotypeIgG1
• Research Areas
  • Neuroscience
  • Neurotransmitter
  • Acetylcholine

Applications

Target

• FunctionTerminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis.
• Tissue specificityIsoform H is highly expressed in erythrocytes.
• Sequence similaritiesBelongs to the type-B carboxylesterase/lipase family.
• Cellular localizationCell membrane; Cell junction > synapse. Secreted. Cell membrane and Nucleus. Only observed in apoptotic nuclei.

Target information above from: UniProt accession P22303 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 43 Human
  • Entrez Gene: 43 Human
  • Entrez Gene: 43 Human
  • Entrez Gene: 83817 Rat
  • Omim: 100740 Human
  • SwissProt: P22303 Human
  • SwissProt: P22303 Human
  • SwissProt: P22303 Human

  • SwissProt: P37136 Rat
  • Unigene: 154495 Human
  • Unigene: 105879 Rat

  see all

• Alternative names
  ACEE antibodyACES_HUMAN antibodyAcetylcholine acetylhydrolase antibody

  Acetylcholinesterase antibodyAcetylcholinesterase YT blood group antibodyACHE antibodyACHE protein antibodyApoptosis related acetylcholinesterase antibodyARACHE antibodyN ACHE antibodyN-ACHE antibodyYT antibodyYt blood group antibody

  see all

Anti-Acetylcholinesterase antibody [AE-2] images

• 

  Flow Cytometry-Anti-Acetylcholinesterase antibody [AE-2](ab77959)
  Overlay histogram showing HeLa cells stained with ab77959 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77959, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.