Overview
- Product nameAnti-M Cadherin [12G4] antibodySee all M Cadherin primary antibodies ...
- DescriptionMouse monoclonal [12G4] to M Cadherin
- SpecificityWe have data to indicate that this antibody may not cross react with Human. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
- Tested applicationsWB, ELISA, ICC, ICC/IF, IHC-Fr more details
- Species reactivityReacts with: Mouse, Cow
- Immunogen
Recombinant extracellular domain of mouse M Cadherin
- Positive controlRIPA lysates from C2C12 cells differentiated into myotubes.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.09% Sodium Azide
Constituents: PBS, Sucrose, PEG -
Concentration information loading... - PuritySize exclusion
- Purification notesab78090 is purified by thiophilic adsorption and size exclusion.
- Clonality Monoclonal
- Clone number12G4
- IsotypeIgG1
- Light chain typekappa
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab78090 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 90 kDa. |
| ELISA | ELISA: Use a concentration of 0.05 µg/ml. |
| ICC | ICC: Use a concentration of 5 µg/ml. |
| ICC/IF | ICC/IF: Use at an assay dependent concentration. |
| IHC-Fr | IHC-Fr: Use at an assay dependent concentration. PubMed: 22606352 |
Target
- RelevanceCadherins are a family of transmembrane glycoproteins that play a key role in Calcium dependent cell-cell adhesion. Several members of the cadherin family have been identified so far, including E- (epithelial), P- (placental), N- (neuronal) and M- (muscle) cadherin. Cadherins consist of an extracellular domain containing 5 cadherin domains, a transmembrane region, and a conserved cytoplasmic domain. Transcripts from this particular cadherin are expressed in myoblasts and upregulated in myotubule-forming cells. M-Cadherin is thought to be essential for the control of morphogenetic processes, specifically myogenesis, and may provide a trigger for terminal muscle cell differentiation.
- Cellular localizationType 1 membrane protein.
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Database links
- Entrez Gene: 12555 Mouse
- SwissProt: P33146 Mouse
- Unigene: 1976 Mouse
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Alternative names
- Cadherin 14 antibodyCadherin 15 antibodyCadherin 3 antibody
- Cadherin14 antibodyCadherin15 antibodyCadherin3 antibodyCCAD antibodyCDH 14 antibodyCDH 15 antibodyCDH 3 antibodyCDH14 antibodyCDH15 antibodyCDH3 antibodyCDHM antibodyMCAD antibodyMRD3 antibodyMuscle cadherin antibodyMyotubule cadherin antibody
see all
Anti-M Cadherin [12G4] antibody images
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![Western blot - Anti-M Cadherin [12G4] antibody (ab78090)](http://a.abcam.com/ps/products/78/ab78090/Images/ab78090-172717-ab78090wb.png)
Western blot - Anti-M Cadherin [12G4] antibody (ab78090)Image courtesy of an an annomous Abreview
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 90 kDaImage courtesy of an an annomous Abreview
Western blot showing ab78090 (1/1000) in 1.5% BSA in TBST pH7.5 labelling M Cadherin. Lanes B1-4 were loaded with 40µg Bovine skeletal muscle tissue lysate, Lane M was loaded with 40µg Mouse skeletal muscle tissue lysate. Left panel: 10 sec exposure; middle panel: 30 sec exposure; right panel: 90 sec exposure.
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![Western blot - M Cadherin antibody [12G4] (ab78090)](http://a.abcam.com/ps/datasheet/Images/78/ab78090/ab78090-1.jpg)
Western blot - M Cadherin antibody [12G4] (ab78090)Anti-M Cadherin [12G4] antibody (ab78090) at 0.5 µg/ml + Lysates of C2C12 cells differentiated into myotubes
Secondary
Goat anti-mouse HRP conjugate
developed using the ECL technique
Predicted band size : 90 kDa
Observed band size : 130 kDa (why is the actual band size different from the predicted?) -
![Immunocytochemistry/ Immunofluorescence - M Cadherin antibody [12G4] (ab78090)](http://a.abcam.com/ps/datasheet/images/78/ab78090/M-Cadherin-Primary-antibodies-ab78090-3.jpg)
Immunocytochemistry/ Immunofluorescence - M Cadherin antibody [12G4] (ab78090)Image courtesy of Ms Marta Przewozniak by Abreview.ab78090 staining M Cadherin in C2C12 murine myoblasts (myotubes) by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton and then blocked using 0.2% BSA, 1% goat serum for 2 hours at 25°C. Samples were then incubated with primary antibody at 1/100 for 12 hours at 4°C. The secondary antibody used was a goat anti-mouse IgG conjugated to Alexa Fluor® 488 (green) used at a 1/200 dilution.
References for Anti-M Cadherin [12G4] antibody (ab78090)
This product has been referenced in:
- Loh KC et al. Sphingosine-1-phosphate enhances satellite cell activation in dystrophic muscles through a S1PR2/STAT3 signaling pathway. PLoS One 7:e37218 (2012). IHC-Fr ; Mouse . Read more (PubMed: 22606352) »
- Kitamoto T & Hanaoka K Notch3 null mutation in mice causes muscle hyperplasia by repetitive muscle regeneration. Stem Cells 28:2205-16 (2010). ICC/IF ; Mouse . Read more (PubMed: 20960513) »
