Anti-S100 antibody [B32.1] - Astrocyte Marker (ab7852)

Overview

• Product nameAnti-S100 antibody [B32.1] - Astrocyte MarkerSee all S100 primary antibodies ...
• Description
  Mouse monoclonal [B32.1] to S100 - Astrocyte Marker
• SpecificityThis antibody reacts with alpha and beta subunits of human S-100 protein (21-24 kD).
• Tested applicationsICC/IF, IHC-P, IHC-FoFr more details
• Species reactivity
  Reacts with: Mouse, Rat, Cow, Human
  
• Immunogen

  Full length native protein (purified) (Cow).

• Positive controlMelanoma tissues containing nerves, such as intestine and pancreas. Brain tissue.

Properties

• FormLiquid
• Storage instructionsStore at +4°C. Do not freeze.
• Storage bufferpH: 7.40
  Preservative: 0.1% Sodium azide
  Constituent: 0.01% PBS
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberB32.1
• IsotypeIgG1
• Light chain typekappa
• Research Areas
  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Intermediate Filaments
  • Class IV
  • Neurofilaments

  • Tags & Cell Markers
  • Cell Type Markers
  • Neuroscience Markers
  • Glial

  • Neuroscience
  • Neurotransmission
  • Calcium Signaling
  • Calcium Binding Proteins

  • Neuroscience
  • Cell Type Marker
  • Glia marker
  • Astrocyte marker

Applications

Target

• FunctionWeakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
• Tissue specificityHighly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
• Sequence similaritiesBelongs to the S-100 family.
  Contains 2 EF-hand domains.
• Cellular localizationCytoplasm.

Target information above from: UniProt accession P23297 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 431787 Cow
  • Entrez Gene: 6271 Human
  • Entrez Gene: 20193 Mouse
  • Entrez Gene: 295214 Rat
  • Omim: 176940 Human
  • SwissProt: P23297 Human
  • SwissProt: P56565 Mouse
  • SwissProt: P35467 Rat

  • Unigene: 515715 Human
  • Unigene: 24662 Mouse
  • Unigene: 11091 Rat

  see all

• Alternative names
  Bpb antibodyNEF antibodyProtein S100-A1 antibody

  S 100 protein alpha chain antibodyS 100 PROTEIN BETA CHAIN antibodyS 100 protein beta subunit antibodyS-100 protein alpha chain antibodyS-100 protein subunit alpha antibodyS100 alpha antibodyS100 beta antibodyS100 beta antibodyS100 calcium binding protein A1 antibodyS100 calcium binding protein B antibodyS100 calcium binding protein beta neural antibodyS100 calcium-binding protein A1 antibodyS100 protein alpha chain antibodyS100 protein alpha polypeptide antibodyS100 protein beta polypeptide neural antibodyS100A antibodyS100a1 antibodyS100B antibodyS100beta antibodyS10A1_HUMAN antibody

  see all

Anti-S100 antibody [B32.1] - Astrocyte Marker images

• 

  Immunohistochemistry (PFA perfusion fixed frozen sections) - S100 antibody [B32.1] - Astrocyte Marker (ab7852)

  ab7852 at a dilution of 1/1000, staining Astrocytes (purple; Alexa 488 secondary at 1/2000) on 30µm coronal brain section tissue in free floating IHC (see protocol link for detailed description). [A] Image taken with 20x objective and [B] Image taken with 40x objective. Images coloured in Photoshop.

  NB: No labeling observed following omission of primary antibody.

  Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).

Protocols

• IHC-FoFr protocol