Anti-Melanoma antibody [HMB45] (ab787)

Overview

• Product nameAnti-Melanoma antibody [HMB45]See all Melanoma primary antibodies ...
• Description
  Mouse monoclonal [HMB45] to Melanoma
• SpecificityThis antibody reacts with a neuraminidase sensitive oligosaccharide side chain of a glycoconjugate present in immature melanosomes. It reacts with junctional and blue nevus cells. Non-melanocytic cells are negative.
• Tested applicationsFlow Cyt, IHC-P, IHC-Fr, ICC/IF more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Extract of pigmented melanoma metastases from lymph nodes.

Properties

• FormLiquid
• Storage instructionsStore at +4°C.
• Storage bufferPreservative: 0.05% Sodium Azide
  Constituents: 1% BSA, Tissue culture supernatant
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberHMB45
• IsotypeIgG1
• Light chain typekappa
• Research Areas
  • Cancer
  • Tumor biomarkers
  • Other

  • Tags & Cell Markers
  • Cell Type Markers
  • Tumor Associated

Applications

Target

• RelevanceMalignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre existing benign nevus, which occurs most often in the skin but also may involve other sites.
• Cellular localizationMembrane; Single-pass membrane protein
• Database links
  • Omim: 605513 Human
  • Omim: 606933 Human
  • SwissProt: Q16655 Human
  • SwissProt: P14679 Human

• Alternative names
  Antigen LB39 AA antibodyAntigen SK29 AA antibodyCMM 1 antibody

  CMM antibodyCMM1 antibodyCutaneous Malignant Melanoma Dysplastic Nevus antibodyDNS antibodyDysplastic Nevus Syndrome antibodyFAMMM antibodyMART1 antibodymelan A antibodyMelan A protein antibodyMelanoma antigen recognized by T-cells 1 antibodyMLM antibodyMonophenol monooxygenase antibodyTumor rejection antigen AB antibodytyrosinase antibody

  see all

Anti-Melanoma antibody [HMB45] images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Melanoma antibody [HMB45] (ab787)

  ab787 - immunohistochemistry

  Formalin fixed paraffin embedded human melanoma stained with ab787, using ABC and AEC chromogen.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Melanoma antibody [HMB45] (ab787)This image is courtesy of an Abreview submitted by Dr. Hongwei Shao
  ab787 staining Melanoma in the human Melanoma cell line WM3248 xenopraph by IHC-P (formaldehyde-fixed paraffin-embedded sections). Tissue samples were fixed with formaldehyde; permeabilized with 0.1% Triton X-100 and blocked with 100% Dakocytomation X0909 for 1 hour at room temperature; antigen retrival was by heat mediation in Citric buffer (pH6). The sample was incubated with primary antibody (1/25) at 4°C for 18 hours. An HRP-conjugated Goat polyclonal to mouse IgG (1/100) was used as secondary antibody.

  See Abreview

• 

  Flow Cytometry-Anti-Melanoma antibody [HMB45](ab787)
  Overlay histogram showing Malme-3 cells stained with ab787 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab787, 1/10 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Malme-3 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.