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Acetyl Coenzyme A carboxylase alpha protein (His tag) (ab79625)

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Overview

  • Product nameAcetyl Coenzyme A carboxylase alpha protein (His tag)See all Acetyl Coenzyme A carboxylase alpha proteins and peptides ...
  • Protein descriptionRecombinant fragment corresponding to amino acids 39-2346 of Human Acetyl Coenzyme A carboxylase alpha with C terminal His tag expressed in a Baculovirus infected Sf9 cell expression system, 266.5kDa. Swiss ID = Q13085
  • Expression hostBaculovirus infected sf9 cells

    • Properties

  • Purification notesPurity: >50% by SDS-PAGE.
  • FormLiquid
  • Storage instructionsStore at -20°C, Stable for 6 months at -20°C
  • Storage bufferPreservative: None
    Constituents: 10% Glycerol, 0.05% Tween 20, 50mM Tris HCl, 3mM DTT, 150mM Sodium chloride, 0.1mM EDTA, 0.1mM EGTA, pH 7.5
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab79625 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
  • Application notesSDS-PAGE: Use at an assay dependent dilution.
    ab79625 is useful for the study of enzyme kinetics, regulation, and to dephosphorylate target substrates.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        ACACACACAACACA
        ACACA_HUMANACC alphaACC-alphaACC1ACC1ACCAacetyl Coenzyme AAcetyl Coenzyme ABiotin carboxylase
      see all
  • FunctionCatalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.
  • Tissue specificityExpressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.
  • PathwayLipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.
  • Involvement in diseaseDefects in ACACA are a cause of acetyl-CoA carboxylase 1 deficiency (ACACAD) [MIM:200350]; also known as ACAC deficiency or ACC deficiency. An inborn error of de novo fatty acid synthesis associated with severe brain damage, persistent myopathy and poor growth.
  • Sequence similaritiesContains 1 ATP-grasp domain.
    Contains 1 biotin carboxylation domain.
    Contains 1 biotinyl-binding domain.
    Contains 1 carboxyltransferase domain.
  • Post-translational
    modifications    Phosphorylation on Ser-1263 is required for interaction with BRCA1.
  • Cellular localizationCytoplasm.

    • Target information above from: UniProt accession Q13085 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    References for Acetyl Coenzyme A carboxylase alpha protein (His tag) (ab79625)

    ab79625 has not yet been referenced specifically in any publications.

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