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Anti-Norovirus GI.1 antibody [P2B2] (ab80028)

Overview

  • Product nameAnti-Norovirus GI.1 antibody [P2B2]
  • Description
    Mouse monoclonal [P2B2] to Norovirus GI.1
  • SpecificitySpecific to Norovirus GI.1. Key epitope is also present on GI.6, and GI.8 capsid proteins.
  • Tested applicationsELISA, WB more details
  • Species reactivity
    Reacts with Norovirus GI.1
  • Immunogen

    A peptide sequence representing an epitope from the Norovirus GI.1 capsid protein.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Proclin300
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • Clonality Monoclonal
  • Clone numberP2B2
  • IsotypeIgG1
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab80028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA ELISA: Use at an assay dependent dilution.
WB WB: 1/400.

Target

  • RelevanceNoroviruses (NoV) are a genetically diverse group of single stranded RNA, nonenveloped viruses belonging to the Caliciviridae family. Noroviruses can genetically be classified into 5 different genogroups (GI, GII, GIII, GIV, and GV) which can be further divided into different genetic clusters or genotypes. For example genogroup II, the most prevalent human genogroup, presently contains 19 genotypes. Genogroups I, II and IV infect humans, whereas genogroup III infects bovine species and genogroup V has recently been isolated in mice. Noroviruses commonly isolated in cases of acute gastroenteritis belong to two genogroups: genogroup I (GI) includes Norwalk virus, Desert Shield virus and Southampton virus and II (GII) which includes Bristol virus, Lordsdale virus, Toronto virus, Mexico virus, Hawaii virus and Snow Mountain virus. Noroviruses contain a positive-sense RNA genome of approximately 7.5 knt, encoding a major structural protein (VP1) of about 58~60 kDa and a minor capsid protein (VP2). The virus particles demonstrate an amorphous surface structure when visualized using electron microscopy and are between 27-38 nm in size.
  • Alternative names
      NoV antibody

References for Anti-Norovirus GI.1 antibody [P2B2] (ab80028)

ab80028 has not yet been referenced specifically in any publications.

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Application Western blot
Sample Pichia pastoris Cell lysate - whole cell (Cells were overexpressing a virus capsid protein)
Loading amount 100 µg
Specification Cells were overexpressing a virus capsid protein
Gel Running Conditions Reduced Denaturing (12% gel, 4% stacking gel)
Blocking step Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
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Submitted May 29 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"