Anti-p60 CAF1 antibody [SS 53] (ab8133)

Overview

• Product nameAnti-p60 CAF1 antibody [SS 53]See all p60 CAF1 primary antibodies ...
• Description
  Mouse monoclonal [SS 53] to p60 CAF1
• Specificityspecific for CAF-1p60.
• Tested applicationsWB, ICC/IF, IP, IHC-P more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Recombinant full length protein (Human).

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferAscitic fluid with 0.1% sodium azide
• Concentration information loading...
• PurityAscites
• Primary antibody notes Chromatin assembly factor 1 (CAF-1) is a member of the histone chaperone family. It is required for inheritance of epigenetically determined chromosomal states, and in vitro, it promotes the assembly of chromatin during DNA replication and DNA repair. CAF-1 is a complex of three subunits; p150, p60, and p48. This complex is localized to the nucleus of human cells. Both the composition and the biochemical activity of CAF-1 are conserved among human, yeast, and Drosophila.
• Clonality Monoclonal
• Clone numberSS 53
• IsotypeIgG1
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Chromatin Remodeling
  • FACT

Applications

Target

• FunctionComplex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. The CCR4-NOT complex functions as general transcription regulation complex.
• Sequence similaritiesBelongs to the WD repeat HIR1 family.
  Contains 7 WD repeats.
• Developmental stageActive complex is found in G1, S and G2 phases.
• Post-translational
  modificationsDifferentially phosphorylated during cell cycle. During mitosis the p60 subunit of inactive CAF-1 is hyperphosphorylated and displaced into the cytosol. Progressivly dephosphorylated from G1 to S and G2 phase. Phosphorylated p60 is recruited to chromatin undergoing DNA repair after UV irradiation in G1, S or G2 phases.
• Cellular localizationNucleus. Cytoplasm. DNA replication foci. Cytoplasmic in M phase.

Target information above from: UniProt accession Q13112 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 8208 Human
  • Omim: 601245 Human
  • SwissProt: Q13112 Human
  • Unigene: 75238 Human

• Alternative names
  CAF 1 subunit B antibodyCAF 1A antibodyCAF I 60 kDa subunit antibody

  CAF IP60 antibodyCAF-1 antibodyCAF-1 antibodyCAF-1 subunit B antibodyCAF-I 60 kDa subunit antibodyCAF-I p60 antibodyCAF1 antibodyCAF1A antibodyCAF1B_HUMAN antibodyCAF1P60 antibodyCHAF 1B antibodyCHAF1B antibodyChromatin assembly factor 1 subunit B antibodyChromatin assembly factor I antibodyChromatin assembly factor I antibodyChromatin assembly factor I p60 subunit antibodyHuman chromatin assembly factor-I p60 subunit antibodyM phase phosphoprotein 7 antibodyM-phase phosphoprotein 7 antibodyMPHOSPH7 antibodyMPP 7 antibodyMPP7 antibodyp60 subunit antibodyp60 subunit antibody

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Anti-p60 CAF1 antibody [SS 53] images

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  Immunocytochemistry/ Immunofluorescence - p60 CAF1 antibody [SS 53] (ab8133)This image is courtesy of an Abreview submitted by Dr Alexander Rapp

  ab8133 staining cultured human HeLa cells by ICC/IF.  Cells were PFA fixed and permeabilized in 0.5% Triton X100 prior to blocking in 5% BSA for 1 hour at 20°C.  The primary antibody was diluted 1/800 and incubated with the sample for 1 hour at 20°C.  A Cy3® conjugated donkey anti-mouse antibody diluted 1/400 was used as the secondary.

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