Anti-Ubiquitin antibody (ab8134)

Overview

• Product nameAnti-Ubiquitin antibodySee all Ubiquitin primary antibodies ...
• Description
  Rabbit polyclonal to Ubiquitin
• SpecificityThis antibody recognizes recognizes ubiquitinated inclusion bodies.
• Tested applicationsELISA, IHC-FoFr, IHC-Fr, IP, IHC-P, WB, ICC/IF more details
• Species reactivity
  Reacts with: Chicken, Cow, Human, Fruit fly (Drosophila melanogaster)
  Predicted to work with: Caenorhabditis elegans
• Immunogen

  Aggregate of purified full length native ubiquitin (Cow) and glutaraldehyde.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• PurityWhole antiserum
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Chromatin Modifying Enzymes
  • Ubiquitylation

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Alzheimer's disease
  • Tangles & Tau

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Other

  • Cell Biology
  • Proteolysis / Ubiquitin
  • Proteasome / Ubiquitin
  • Ubiquitin

Applications

Target

• RelevanceFunction: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Similarity: Belongs to the ubiquitin family. Contains 3 ubiquitin-like domains.
• Cellular localizationCell Membrane, Cytoplasmic and Nuclear
• Database links
  • Entrez Gene: 281370 Cow
  • Entrez Gene: 38456 Fruit fly (Drosophila melanogaster)
  • Entrez Gene: 38456 Fruit fly (Drosophila melanogaster)
  • Entrez Gene: 38456 Fruit fly (Drosophila melanogaster)
  • Entrez Gene: 38456 Fruit fly (Drosophila melanogaster)
  • Entrez Gene: 38456 Fruit fly (Drosophila melanogaster)
  • Entrez Gene: 38456 Fruit fly (Drosophila melanogaster)
  • Entrez Gene: 38456 Fruit fly (Drosophila melanogaster)

  • Entrez Gene: 38456 Fruit fly (Drosophila melanogaster)
  • Entrez Gene: 7314 Human
  • Entrez Gene: 7314 Human
  • Entrez Gene: 7314 Human
  • Entrez Gene: 7314 Human
  • Entrez Gene: 7314 Human
  • Entrez Gene: 7314 Human
  • Entrez Gene: 7314 Human
  • Entrez Gene: 7314 Human
  • Omim: 191339 Human
  • SwissProt: P0CG53 Cow
  • SwissProt: P68198 Fruit fly (Drosophila melanogaster)
  • SwissProt: P0CG69 Fruit fly (Drosophila melanogaster)
  • SwissProt: P68198 Fruit fly (Drosophila melanogaster)
  • SwissProt: P0CG69 Fruit fly (Drosophila melanogaster)
  • SwissProt: P68198 Fruit fly (Drosophila melanogaster)
  • SwissProt: P0CG69 Fruit fly (Drosophila melanogaster)
  • SwissProt: P68198 Fruit fly (Drosophila melanogaster)
  • SwissProt: P0CG69 Fruit fly (Drosophila melanogaster)
  • SwissProt: P68198 Fruit fly (Drosophila melanogaster)
  • SwissProt: P0CG69 Fruit fly (Drosophila melanogaster)
  • SwissProt: P68198 Fruit fly (Drosophila melanogaster)
  • SwissProt: P0CG69 Fruit fly (Drosophila melanogaster)
  • SwissProt: P68198 Fruit fly (Drosophila melanogaster)
  • SwissProt: P0CG69 Fruit fly (Drosophila melanogaster)
  • SwissProt: P68198 Fruit fly (Drosophila melanogaster)
  • SwissProt: P0CG69 Fruit fly (Drosophila melanogaster)
  • SwissProt: P62988 Human
  • SwissProt: P0CG48 Human
  • SwissProt: P0CG47 Human
  • SwissProt: P62988 Human
  • SwissProt: P0CG48 Human
  • SwissProt: P0CG47 Human
  • SwissProt: P62988 Human
  • SwissProt: P0CG48 Human
  • SwissProt: P0CG47 Human
  • SwissProt: P62988 Human
  • SwissProt: P0CG48 Human
  • SwissProt: P0CG47 Human
  • SwissProt: P62988 Human
  • SwissProt: P0CG48 Human
  • SwissProt: P0CG47 Human
  • SwissProt: P62988 Human
  • SwissProt: P0CG48 Human
  • SwissProt: P0CG47 Human
  • SwissProt: P62988 Human
  • SwissProt: P0CG48 Human
  • SwissProt: P0CG47 Human
  • SwissProt: P62988 Human
  • SwissProt: P0CG48 Human
  • SwissProt: P0CG47 Human
  • Unigene: 2641 Fruit fly (Drosophila melanogaster)
  • Unigene: 2641 Fruit fly (Drosophila melanogaster)
  • Unigene: 2641 Fruit fly (Drosophila melanogaster)
  • Unigene: 2641 Fruit fly (Drosophila melanogaster)
  • Unigene: 2641 Fruit fly (Drosophila melanogaster)
  • Unigene: 2641 Fruit fly (Drosophila melanogaster)
  • Unigene: 2641 Fruit fly (Drosophila melanogaster)
  • Unigene: 2641 Fruit fly (Drosophila melanogaster)
  • Unigene: 356190 Human
  • Unigene: 520348 Human
  • Unigene: 356190 Human
  • Unigene: 520348 Human
  • Unigene: 356190 Human
  • Unigene: 520348 Human
  • Unigene: 356190 Human
  • Unigene: 520348 Human
  • Unigene: 356190 Human
  • Unigene: 520348 Human
  • Unigene: 356190 Human
  • Unigene: 520348 Human
  • Unigene: 356190 Human
  • Unigene: 520348 Human
  • Unigene: 356190 Human
  • Unigene: 520348 Human

  see all

• Alternative names
  FLJ25987 antibodyMGC8385 antibodyPolyubiquitin B antibody

  RPS 27A antibodyRPS27A antibodyUBA 52 antibodyUBA 80 antibodyUBA52 antibodyUBA80 antibodyUBB antibodyUBB_HUMAN antibodyUBC antibodyUBCEP 1 antibodyUBCEP 2 antibodyUBCEP1 antibodyUBCEP2 antibodyUbiquitin antibodyubiquitin B antibody

  see all

Anti-Ubiquitin antibody images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Ubiquitin antibody (ab8134)
  Ab8134 staining human normal testis. Staining is localised to the nucleus and cytoplasm.
  Left panel: with primary antibody at a dilution of 1:1000. Right panel: isotype control.
  Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the DAKO 3-in-1 antigen retrieval buffer citrate pH 6.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
• 

  Immunocytochemistry/ Immunofluorescence-Ubiquitin antibody(ab8134)
  ICC/IF image of ab8134 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8134, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.