Overview
- Product nameAnti-RSPO1 antibody [7A6]See all RSPO1 primary antibodies ...
- DescriptionMouse monoclonal [7A6] to RSPO1
- Tested applicationsWB, ELISA more details
- Species reactivityReacts with: Human
- Immunogen
Purified recombinant fragment of human RSPO1 expressed in E.coli.
- Positive controlRecombinant RSPO1; RSPO1 (aa21-263)-hIgGFc transfected HEK293 cell lysate.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.03% Sodium Azide
Constituents: Ascites -
Concentration information loading... - PurityAscites
- Clonality Monoclonal
- Clone number7A6
- IsotypeIgG1
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab81600 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/500 - 1/2000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). |
| ELISA | ELISA: 1/10000. |
Target
- FunctionActivator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway, possibly via a direct interaction with Wnt proteins, and in a Wnt-independent beta catenin pathway through a receptor signaling pathway that may not use frizzled/LRP receptors. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination.
- Tissue specificityAbundantly expressed in adrenal glands, ovary, testis, thyroid and trachea but not in bone marrow, spinal cord, stomach, leukocytes colon, small intestine, prostate, thymus and spleen.
- Involvement in diseaseDefects in RSPO1 are the cause of palmoplantar keratoderma with squamous cell carcinoma of skin and sex reversal (PKKSCC) [MIM:610644]. This recessive syndrome is characterized by XX (female to male) SRY-independent sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin.
- Sequence similaritiesBelongs to the R-spondin family.
Contains 2 FU (furin-like) repeats.
Contains 1 TSP type-1 domain. - DomainThe FU repeats are required for activation and stabilization of beta-catenin.
- Cellular localizationSecreted.
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Database links
- Entrez Gene: 284654 Human
- Omim: 609595 Human
- SwissProt: Q2MKA7 Human
- Unigene: 135015 Human
Target information above from: UniProt accession
Q2MKA7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- CRISTIN3 antibodyFLJ40906 antibodyhRspo1 antibody
- R spondin homolog (Xenopus laevis) antibodyR spondin homolog antibodyR spondin1 antibodyR-spondin homolog (Xenopus laevis) antibodyR-spondin-1 antibodyR-spondin1 antibodyRoof plate specific spondin antibodyroof plate-specific spondin antibodyRoof plate-specific spondin-1 antibodyRP11-566C13.1 antibodyRSPO antibodyRspo1 antibodyRSPO1_HUMAN antibody
see all
Anti-RSPO1 antibody [7A6] images
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![Western blot - RSPO1 antibody [7A6] (ab81600)](http://a.abcam.com/ps/datasheet/images/81/ab81600/RSPO1-Primary-antibodies-ab81600-1.jpg)
Western blot - RSPO1 antibody [7A6] (ab81600)All lanes : Anti-RSPO1 antibody [7A6] (ab81600) at 1/500 dilution
Lane 1 : Recombinant RSPO1
Lane 2 : RSPO1 (aa21-263)-hIgGFc transfected HEK293 cell lysate
Predicted band size : 29 kDa
Observed band size : 29,70 kDa (why is the actual band size different from the predicted?)
References for Anti-RSPO1 antibody [7A6] (ab81600)
ab81600 has not yet been referenced specifically in any publications.
