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Anti-RSPO1 antibody [7A6] (ab81600)

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Overview

  • Product nameAnti-RSPO1 antibody [7A6]See all RSPO1 primary antibodies ...
  • Description
    Mouse monoclonal [7A6] to RSPO1
  • Tested applicationsWB, ELISA more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified recombinant fragment of human RSPO1 expressed in E.coli.

  • Positive controlRecombinant RSPO1; RSPO1 (aa21-263)-hIgGFc transfected HEK293 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab81600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/500 - 1/2000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
ELISA ELISA: 1/10000.

Target

  • FunctionActivator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway, possibly via a direct interaction with Wnt proteins, and in a Wnt-independent beta catenin pathway through a receptor signaling pathway that may not use frizzled/LRP receptors. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination.
  • Tissue specificityAbundantly expressed in adrenal glands, ovary, testis, thyroid and trachea but not in bone marrow, spinal cord, stomach, leukocytes colon, small intestine, prostate, thymus and spleen.
  • Involvement in diseaseDefects in RSPO1 are the cause of palmoplantar keratoderma with squamous cell carcinoma of skin and sex reversal (PKKSCC) [MIM:610644]. This recessive syndrome is characterized by XX (female to male) SRY-independent sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin.
  • Sequence similaritiesBelongs to the R-spondin family.
    Contains 2 FU (furin-like) repeats.
    Contains 1 TSP type-1 domain.
  • DomainThe FU repeats are required for activation and stabilization of beta-catenin.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession Q2MKA7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        CRISTIN3 antibodyFLJ40906 antibodyhRspo1 antibody
        R spondin homolog (Xenopus laevis) antibodyR spondin homolog antibodyR spondin1 antibodyR-spondin homolog (Xenopus laevis) antibodyR-spondin-1 antibodyR-spondin1 antibodyRoof plate specific spondin antibodyroof plate-specific spondin antibodyRoof plate-specific spondin-1 antibodyRP11-566C13.1 antibodyRSPO antibodyRspo1 antibodyRSPO1_HUMAN antibody
      see all

    Anti-RSPO1 antibody [7A6] images

    • Western blot - RSPO1 antibody [7A6] (ab81600)
      Western blot - RSPO1 antibody [7A6] (ab81600)
      All lanes : Anti-RSPO1 antibody [7A6] (ab81600) at 1/500 dilution

      Lane 1 : Recombinant RSPO1
      Lane 2 : RSPO1 (aa21-263)-hIgGFc transfected HEK293 cell lysate


      Predicted band size : 29 kDa
      Observed band size : 29,70 kDa (why is the actual band size different from the predicted?)

    References for Anti-RSPO1 antibody [7A6] (ab81600)

    ab81600 has not yet been referenced specifically in any publications.

    Publishing research using ab81600 ? Please let us know so that we can cite the reference in this datasheet

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    Customer kindly contacted us to inquire about the immunogen sequence used for this product.

    Thank you for contacting us.

    I wish to apologize for the delay in attaining information about this immunogen for you. I have been in contact with the lab on your behalf.Unfortunately, we do not have the information of the amino acid range or se...

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