Anti-TATA binding protein TBP antibody [1TBP18] - Nuclear Loading Control and ChIP Grade (ab818)

Overview

• Product nameAnti-TATA binding protein TBP antibody [1TBP18] - Nuclear Loading Control and ChIP GradeSee all TATA binding protein TBP primary antibodies ...
• Description
  Mouse monoclonal [1TBP18] to TATA binding protein TBP - Nuclear Loading Control and ChIP Grade
• Tested applicationsChIP/Chip, ELISA, IHC-P, EMSA, IP, WB, Flow Cyt, ChIP, ICC/IF, ICC, IHC-Fr more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  

  Does not react with

  Saccharomyces cerevisiae, Xenopus laevis, Fruit fly (Drosophila melanogaster), Silk worm
• Immunogen

  Synthetic peptide (Human).

• EpitopeWithin amino acid residues 1-20 of human, mouse and rat TBP. The specific epitope for this antibody is accessible when the C-terminal domain of TBP is removed or when TBP is complexed with DNA.
• Positive controlHeLa nuclear extract

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPBS with 0.02% sodium azide
• Concentration information loading...
• PurityProtein G purified
• Clonality Monoclonal
• Clone number1TBP18
• IsotypeIgG1
• Research Areas
  • Isotype/Loading Controls
  • Loading Controls
  • TBP - Nuclear

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Polymerase associated factors
  • Pol II Transcription
  • TFIID

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Preinitiation complex

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Polymerase associated factors
  • Pol II Transcription
  • TAFs

  • Epigenetics and Nuclear Signaling
  • ChIP'ing antibodies
  • ChIP'ing antibodies

Applications

Target

• FunctionGeneral transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA.
• Tissue specificityWidely expressed, with levels highest in the testis and ovary.
• Involvement in diseaseDefects in TBP are the cause of spinocerebellar ataxia type 17 (SCA17) [MIM:607136]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA17 is an autosomal dominant cerebellar ataxia (ADCA) characterized by widespread cerebral and cerebellar atrophy, dementia and extrapyramidal signs. The molecular defect in SCA17 is the expansion of a CAG repeat in the coding region of TBP. Longer expansions result in earlier onset and more severe clinical manifestations of the disease.
• Sequence similaritiesBelongs to the TBP family.
• Cellular localizationNucleus.

Target information above from: UniProt accession P20226 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 6908 Human
  • Entrez Gene: 21374 Mouse
  • Entrez Gene: 117526 Rat
  • Omim: 600075 Human
  • SwissProt: P20226 Human
  • SwissProt: P29037 Mouse
  • SwissProt: Q66HB1 Rat
  • Unigene: 590872 Human

  • Unigene: 244820 Mouse

  see all

• Alternative names
  GTF2D antibodyGTF2D1 antibodyMGC117320 antibody

  MGC126054 antibodyMGC126055 antibodySCA 17 antibodySCA17 antibodyTATA binding factor antibodyTATA box binding protein antibodyTATA box factor antibodyTATA sequence binding protein antibodyTATA sequence-binding protein antibodyTATA-binding factor antibodyTATA-box factor antibodyTATA-box-binding protein antibodyTBP antibodyTBP_HUMAN antibodyTF2D antibodyTFIID antibodyTranscription initiation factor TFIID TBP subunit antibody

  see all

Anti-TATA binding protein TBP antibody [1TBP18] - Nuclear Loading Control and ChIP Grade images

• 

  Western blot - TATA binding protein TBP antibody [1TBP18] -(ab818)
  Anti-TATA binding protein TBP antibody [1TBP18] - Nuclear Loading Control and ChIP Grade (ab818) at 1/2000 dilution + Ros C cells with endogenous TBP
  
  Performed under reducing conditions.
  
  Predicted band size : 38 kDa
  
• 

  ChIP - TATA binding protein TBP antibody [1TBP18] - ChIP Grade (ab818)

  Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 µg of chromatin, 8 µg of  ab818 (blue), and 20 µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The last wash was performed with final wash buffer containing 250 mM NaCl. The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes are located in the core promoter region of the genes.
• 

  ELISA - TATA binding protein TBP antibody [1TBP18] - Nuclear Loading Control and ChIP Grade (ab818)This image is a courtesy of Anonymous Abreview

  ab818 staining TATA binding protein TBP by ELISA. The sample was purified from human AGS gastric carcinoma cell line and blocked with 5% BSA for 1 hour at 25°C. The primary antibody was used at 1/1000 dilution, and incubated with sample for 16 hour at 4°C. ab6729 AP conjugated rabbit polyclonal to mouse IgG was used as secondary, diluted at 1/1000.

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• 

  Western blot - TATA binding protein TBP antibody [1TBP18] - Nuclear Loading Control and ChIP Grade (ab818)
  All lanes : Anti-TATA binding protein TBP antibody [1TBP18] - Nuclear Loading Control and ChIP Grade (ab818) at 1/2000 dilution
  
  Lane 1 : Human Huh7 nuclear cell lysate
  Lane 2 : Human Huh7 cytoplast cell lysate
  Lane 3 : Human HepG2 nuclear cell lysate
  Lane 4 : Human HepG2 cytoplast cell lysate
  
  Lysates/proteins at 40 µg per lane.
  
  Secondary
  HRP-conjugated goat polyclonal to mouse IgG at 1/10000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 38 kDa
  Observed band size : 38 kDa
  
  
  Exposure time : 30 seconds

  This image is a courtesy of Chien Hsin Lee

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• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TATA binding protein TBP antibody [1TBP18] - Nuclear Loading Control and ChIP Grade (ab818)This image is courtesy of anonymous Abreview.

  ab818 staining TATA binding protein TBP in Human stomach tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The tissue underwent formaldehyde fixation before heat mediated antigen retrieval in 10mM Citrate buffer pH 6.0. Blocking was done in 5% serum for 1 hour at 23°C. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 23°C. An HRP-conjugated Goat polyclonal to Mouse IgG was used undiluted as the secondary antibody..

   

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• 

  Immunocytochemistry/ Immunofluorescence - TATA binding protein TBP antibody [1TBP18] - Nuclear Loading Control and ChIP Grade (ab818)Image courtesy of an anonymous Abreview.
  ab818 staining TATA binding protein TBP in NIH3T3 mouse fibroblast cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in formaldehyde, permeabilised in 0.025% Triton X and then blocked using 5% serum for 1 hour at 23°C. Samples were then incubated with primary antibody at 1/200 for 16 hours at 4°C. The secondary antibody used was a goat anti-mouse IgG conjugated to Alexa Fluor® 350 (blue) used at a 1/1000 dilution.

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• 

  Flow Cytometry-TATA binding protein TBP antibody [1TBP18] - Nuclear Loading Control and ChIP Grade(ab818)
  Overlay histogram showing HeLA cells stained with ab818 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab818, 1µg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.