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KAT2B / PCAF protein (Human) (ab82074)

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Overview

  • Product nameKAT2B / PCAF protein (Human)See all KAT2B / PCAF proteins and peptides ...
  • Protein descriptionRecombinant full length human KAT2B / PCAF expressed in baculovirus system; amino acids 1-832, 93kDa. Swiss ID = Q92831
  • Expression hostBaculovirus

    • Properties

  • Purification notesab82074 is purified by an affinity column in combination with FPLC chromatography and is greater than 95% homogeneous. ab82074 contains no detectable proteases, DNase and RNase activity.
  • Biological activity1 unit equals 1 nanogram of purified protein.
  • FormLiquid
  • Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 20% Glycerol, 20mM Tris HCl, 100mM Potassium chloride, 1mM DTT, 0.2mM EDTA, pH 8
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab82074 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Gel supershift assays
  • Application notesGSA: Use 1-5 units in a 20µl reaction.
    Protein-protein interaction assay: Use 100-200 units.
    in vitro transcription assay: Use 50-100 units.
    in vitro acetylation assay: Use 100-200 units.
    Cell growth assay: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        CAFCREBBP associated factorGCN5
        GCN5LGCN5L1Histone acetylase PCAFHistone acetyltransferase KAT2BHistone acetyltransferase PCAFK(lysine) acetyltransferase 2BKAT2BKAT2B_HUMANLysine acetyltransferase 2BPP/CAFp300/CBP associated factorP300/CBP-associated factorPcaf
      see all
  • FunctionFunctions as a histone acetyltransferase (HAT) to promote transcriptional activation. Has significant histone acetyltransferase activity with core histones (H3 and H4), and also with nucleosome core particles. Inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes.
  • Tissue specificityUbiquitously expressed but most abundant in heart and skeletal muscle.
  • Sequence similaritiesBelongs to the GCN5 family.
    Contains 1 bromo domain.
    Contains 1 N-acetyltransferase domain.
  • DomainThe bromodomain mediates binding to HIV-1 Tat.
  • Cellular localizationNucleus.

    • Target information above from: UniProt accession Q92831 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    References for KAT2B / PCAF protein (Human) (ab82074)

    ab82074 has not yet been referenced specifically in any publications.

    Publishing research using ab82074 ? Please let us know so that we can cite the reference in this datasheet

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    Submit a Question for ab82074

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    Is the enzyme (PCAF) catalytically active on histone or assembled nucleosome?

    We don't have any in-house functional testing data to share unfortunately. However, here are some further details regarding ab82074 KAT2B / PCAF protein (Human):

    Applications:

    Recombinant PCAF can be used for: 1) protein-protein intera...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"