Anti-beta Actin [mAbcam 8226] antibody (ab8226)

Overview

• Product nameAnti-beta Actin [mAbcam 8226] antibodySee all beta Actin primary antibodies ...
• Description
  Mouse monoclonal [mAbcam 8226] to beta Actin
• SpecificityDoes not cross-react with adult cardiac, smooth, or skeletal muscle actin.
• Tested applicationsICC/IF, ICC, Flow Cyt, IHC-FrFl, IHC-P, IHC-Fr, IP, WB more details
• Species reactivity
  Reacts with: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Dog, Human, Pig, African Green Monkey, Chinese Hamster, Armenian Hamster
  Predicted to work with: Sheep, Guinea pig
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human beta Actin.

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab13772.)

• General notesThe concentration of this antibody is batch dependent.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferpH: 7.40
  Preservative: 0.02% Sodium azide
  Constituent: PBS
  Note: Contains 0.4M Arginine
• Concentration information loading...
• PurityIgG fraction
• Clonality Monoclonal
• Clone numbermAbcam 8226
• MyelomaSp2/0-Ag14
• IsotypeIgG1
• Light chain typekappa
• Research Areas
  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Microfilaments
  • Actin etc
  • Actin

  • Isotype/Loading Controls
  • Loading Controls
  • Beta Actin

  • Tags & Cell Markers
  • Subcellular Markers
  • Cytoskeleton
  • Actin

Applications

Target

• FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
• Involvement in diseaseDefects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
• Sequence similaritiesBelongs to the actin family.
• Post-translational
  modificationsISGylated.
• Cellular localizationCytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Target information above from: UniProt accession P60709 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 60 Human
  • Entrez Gene: 11461 Mouse
  • Entrez Gene: 100009272 Rabbit
  • Entrez Gene: 81822 Rat
  • Omim: 102630 Human
  • SwissProt: P60706 Chicken
  • SwissProt: P60712 Cow
  • SwissProt: P60708 Horse

  • SwissProt: P60709 Human
  • SwissProt: P60710 Mouse
  • SwissProt: P29751 Rabbit
  • SwissProt: P60711 Rat
  • SwissProt: P60713 Sheep
  • Unigene: 520640 Human
  • Unigene: 708120 Human
  • Unigene: 727576 Human
  • Unigene: 328431 Mouse
  • Unigene: 391967 Mouse
  • Unigene: 94978 Rat

  see all

• Alternative names
  A X actin like protein antibodyACTB antibodyACTB_HUMAN antibody

  Actin antibodyActin cytoplasmic 1 antibodyActx antibodyb-actin antibodyBeta cytoskeletal actin antibodyBeta-actin antibodycytoplasmic 1 antibodyMelanoma X actin antibodyN-terminally processed antibodyPS1TP5 binding protein 1 antibodyPS1TP5BP1 antibody

  see all

Anti-beta Actin [mAbcam 8226] antibody images

• 

  Western blot - beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)
  Lanes 1 - 3 : Anti-beta Actin [mAbcam 8226] antibody (ab8226) at 1 µg/ml (5% BSA BLOCK)
  Lanes 4 - 6 : Anti-beta Actin [mAbcam 8226] antibody (ab8226) at 1 µg/ml (5% MILK BLOCK)
  
  Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
  Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
  Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
  Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
  Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
  Lane 6 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size : 42 kDa
  Observed band size : 42 kDa
  
  
  Exposure time : 8 minutes
• 

  Immunofluorescence - beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)

  Immunofluorescence using ab8226 at 5ug/ml incubated for 1 hour on Rat Colon Cancer cells.

  Cells were fixed with ice-cold methanol for 5 mins, then for all following steps, permeabilised in TBS-T for 30 mins, blocked with 5% BSA for 30 mins and then washed in TBS-T. Secondary antibody was Alexa Fluor 488 goat anti-mouse IgG at 1/1000 incubated for 1 hour. Cells were counterstained with DAPI. Image at 400X magnification. All incubations were at room temperature.

  The beta actin fibres can be seen arrayed around the edge of the cells.

• 

  Western blot - beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)
  Anti-beta Actin [mAbcam 8226] antibody (ab8226) at 0.5 µg/ml + HeLa cell lysate
  
  Secondary
  Goat polyclonal to mouse IgG H&L (HRP) at 1/5000 dilution
  
  Performed under non-reducing conditions.
  
  Predicted band size : 42 kDa
  Observed band size : 42 kDa
  
  
  Exposure time : 30 seconds
• 

  Immunocytochemistry - beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)This image is a courtesy of Anonymous Abreview

  ab8226 staining beta Actin in human AGS cells by Immunocytochemistry. Cells were fixed with formaldehyde, permeabilized with 0.025% Triton-X in TBS and blocking with 5% serum was performed for 1 hour at 23⁰C. Samples were incubated with primary antibody (1/500) for 1 hour at 23°C. An HRP^®-conjugated goat polyclonal to mouse IgG was used undiluted as secondary antibody.

  See Abreview

• 

  Immunocytochemistry/ Immunofluorescence - beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)This image is a courtesy of Anonymous Abreview

  ab8226 staining beta Actin in human gastric epithelial AGS cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in formaldehyde. Permeabilization and blocking was carried out using 5% BSA containing 0.025% Triton X in TBS for 1 hour at 23°C. Primary antibody was used at 5µg/ml for 1 hour at 23°C. An Alexa Fluor 488^® conjugated goat polyclonal to mouse IgG was used as the secondary antibody at a 1/1000 dilution.

  See Abreview

• 

  Western blot - Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)
  
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 42 kDa
  
  
  Exposure time : 10 seconds
• 

  Western blot - beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)
  Lane 1 : Anti-beta Actin [mAbcam 8226] antibody (ab8226) at 1/1000 dilution
  Lane 2 : Anti-beta Actin [mAbcam 8226] antibody (ab8226) at 1/10000 dilution
  Lanes 3 - 4 : Anti-beta Actin [mAbcam 8226] antibody (ab8226) at 1/500 dilution
  
  Lane 1 : HeLa Cell lysate
  Lane 2 : HeLa Cell lysate
  Lane 3 : 293 Cell lysate
  Lane 4 : 3T3 Mouse Cell lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6728) at 1/5000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size : 42 kDa
  
  
  Exposure time : 10 seconds