Overview
- Product nameAnti-LMX1b antibodySee all LMX1b primary antibodies ...
- DescriptionRabbit polyclonal to LMX1b
- Tested applicationsELISA, WB more details
- Species reactivityReacts with: Mouse, Human
- Immunogen
3 synthetic peptides (human, LMX1b isoform) conjugated to KLH. Protein length 372 aa. First peptide is close to the N-terminal between 1-60 aa. The second and third peptides are close to the C-terminal between 241-372 aa.
- Positive controlInduced culture of E.coli (one shot Top10 competent cells).
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
Constituents: Whole serum -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesCrude serum, final bleed.
- Clonality Polyclonal
- IsotypeIgG
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Research Areas
Applications
Our Abpromise guarantee covers the use of ab82835 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ELISA | |
| WB |
Antibody specificity was verified by direct ELISA against the 3 immunogen peptides. A minimum titer of 1/70000 is determined for one of the three peptides.
WB: 1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
- FunctionEssential for the specification of dorsal limb fate at both the zeugopodal and autopodal levels.
- Tissue specificityExpressed in most tissues. Highest levels in testis, thyroid, duodenum, skeletal muscle, and pancreatic islets.
- Involvement in diseaseDefects in LMX1B are the cause of nail-patella syndrome (NPS) [MIM:161200]; also known as onychoosteodysplasia. NPS is a disease that cause abnormal skeletal patterning and renal dysplasia.
- Sequence similaritiesContains 1 homeobox DNA-binding domain.
Contains 2 LIM zinc-binding domains. - Cellular localizationNucleus.
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Database links
- Entrez Gene: 4010 Human
- Entrez Gene: 16917 Mouse
- GenBank: U77457.1 Human
- Omim: 602575 Human
- SwissProt: O60663 Human
- SwissProt: O88609 Mouse
- Unigene: 129133 Human
- Unigene: 39825 Mouse
Target information above from: UniProt accession
O60663
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- LIM homeo box transcription factor 1 beta antibodyLIM homeobox transcription factor 1 beta antibodyLIM homeobox transcription factor 1-beta antibody
- LIM-homeobox protein 1.2 antibodyLIM/homeobox protein 1.2 antibodyLIM/homeobox protein LMX1B antibodyLMX 1.2 antibodyLMX-1.2 antibodyLMX1.2 antibodyLMX1B antibodyLMX1B_HUMAN antibodyMGC138325 antibodyMGC142051 antibodyNPS 1 antibodyNPS1 antibody
see all
Anti-LMX1b antibody images
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Western blot - LMX1b antibody (ab82835)All lanes : Anti-LMX1b antibody (ab82835) at 1/1000 dilution
Lane 1 : uninduced culture of E.coli (one shot Top10 competent cells)
Lane 2 : induced culture of E.coli (one shot Top10 competent cells)
Secondary
anti-Rabbit IgG HRP at 1/1000 dilution
Predicted band size : 56 kDa
Observed band size : 56 kDa
References for Anti-LMX1b antibody (ab82835)
ab82835 has not yet been referenced specifically in any publications.
