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Overview

  • Product nameAnti-IZUMO1 antibodySee all IZUMO1 primary antibodies ...
  • Description
    Rabbit polyclonal to IZUMO1
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 301-350 (SLALITGLTF AIFRRRKVID FIKSSLFGLG SGAAEQTQVP KEKATDSRQQ) of human IZUMO1 (NP_872381).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive controlFetal heart lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab82887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
  • Application notesELISA titre using peptide based assay, 1:62500.
    WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionInvolved in the fertilization process. May be involved in the fusion of the sperm with the egg.
    • Tissue specificitySperm-specific (at protein level). Detectable on sperm surface only after the acrosome reaction.
    • Sequence similaritiesBelongs to the Izumo family.
      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    • Post-translational
      modifications      N-glycosylated. Glycosylation is not essential for fusion and for proper protein trafficking in sperm.
      Phosphorylated.
    • Cellular localizationMembrane.

      • Target information above from: UniProt accession Q8IYV9 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      • Alternative names
          FLJ61440 antibodyIZUM1_HUMAN antibodyIZUMO antibody
          Izumo sperm egg fusion 1 antibodyIzumo sperm-egg fusion protein 1 antibodyIzumo1 antibodyMGC34799 antibodyOBF antibodyOocyte binding/fusion factor antibodySperm specific protein izumo antibodySperm-specific protein izumo antibody
        see all

      Anti-IZUMO1 antibody images

      • Western blot - IZUMO1 antibody (ab82887)
        Western blot - IZUMO1 antibody (ab82887)
        Anti-IZUMO1 antibody (ab82887) at 1 µg/ml + Fetal heart lysate at 10 µg

        Secondary
        HRP conjugated anti-Rabbit IgG at 1/50000 dilution

        Predicted band size : 39 kDa
        Observed band size : 39 kDa

      References for Anti-IZUMO1 antibody (ab82887)

      ab82887 has not yet been referenced specifically in any publications.

      Publishing research using ab82887 ? Please let us know so that we can cite the reference in this datasheet

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"