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ATP Assay Kit (Colorimetric/Fluorometric) (ab83355)

Q&A (72)Specific References (8)

Overview

  • Product nameATP Assay Kit (Colorimetric/Fluorometric)See all ATP kits ...
  • Tests
    100 x 1 assay
  • Sample type
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay typeQuantitative
  • Sensitivity
    < 1 µM
  • Assay time
    1h 00m
  • Species reactivity
    Reacts with: Human
    Predicted to work with: all Mammals
  • Product overview

    Abcam's ATP Assay Kit (Colorimetric/Fluorometric) is designed to be a robust, simple method which utilizes the phosphorylation of glycerol to generate a product that is easily quantified by colorimetric (λmax = 570 nm) or fluorometric (Ex/Em = 535/587 nm) methods.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    ATP is the primary energy currency of living systems. Virtually all energy requiring processes utilize the chemical energy stored in the phosphate bond of ATP. ATP is formed exclusively in the mitochondria and a variety of genetic diseases can affect ATP formation in the mitochondria.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceATP (Adenosine-5'-triphosphate) is a multifunctional nucleotide that is most important in intracellular energy transfer. Cell death (especially apoptosis) is an energy-dependent process that requires ATP. As ATP levels fall to a point where the cell can no longer perform basic metabolic functions, the cell will die. A typical apoptotic cell exhibits a significant decrease in ATP level. Therefore, loss of ATP level in cells has been used as an indicator of cell death. In contrast, cell proliferation has been recognized by increased levels of ATP.
    • Alternative names
        Adenosine 5' triphosphate

    Applications

    Our Abpromise guarantee covers the use of ab83355 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    ATP Assay Kit (Colorimetric/Fluorometric) images

    • Functional Studies - ATP Colorimetric/Fluorometric Assay Kit (ab83355)
      Functional Studies - ATP Colorimetric/Fluorometric Assay Kit (ab83355)
      Example of colorimetric ATP assay standard curve.
    • Functional Studies - ATP Colorimetric/Fluorometric Assay Kit (ab83355)
      Functional Studies - ATP Colorimetric/Fluorometric Assay Kit (ab83355)
      Example of fluorometric ATP assay standard curve.

    Protocols

    References for ATP Assay Kit (Colorimetric/Fluorometric) (ab83355)

    This product has been referenced in:
    • Mayeur S  et al. Maternal calorie restriction modulates placental mitochondrial biogenesis and bioenergetic efficiency: putative involvement in fetoplacental growth defects in rats. Am J Physiol Endocrinol Metab 304:E14-22 (2013). Functional Studies . Read more (PubMed: 23092912) »
    • Patel VA  et al. Recognition-dependent Signaling Events in Response to Apoptotic Targets Inhibit Epithelial Cell Viability by Multiple Mechanisms: IMPLICATIONS FOR NON-IMMUNE TISSUE HOMEOSTASIS. J Biol Chem 287:13761-77 (2012). Read more (PubMed: 22396534) »

    See all 8 Publications for this product

    Publishing research using ab83355 ? Please let us know so that we can cite the reference in this datasheet

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    Submit a Question for ab83355

    Displaying 1 - 10 of 72 results for Q&A

    10
    Votes: Highest First



    Inquiry: Re: ATP Assay Kit (Colorimetric/Fluorometric) Do you have any recommendations for the type of 96 well plate to use for the fluorometric assay? Black, flat bottomed, low/high binding? Thank you.

    Thank you for contacting us.

    For the colorimetric assay, please use a clear plate. For the fluorometric assay a clear or a white plate can be used. Use flat bottom plates.

    I hope this information is helpful to you. Please do not hesitate...

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    I want to use ATP Assay Kit to determine the ATP level in bone marrow cells. My quesation is the ATP level measured by this kit is total ATP (cellular plus inside mitochondria) or only cellular ATP? Dose the ATP assay Buffer lysis both cell membrane and m...

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    This kit will detects Total ATP.


    I would like to use the ATP assay kit to measure ATP levels in skeletal muscle.
    Could you please give me an appropriate protocol for sample preparation?
    I see in the protocol of the kit that tissue has to be lysed in the ATP assay buffer of t...

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    Yes, you will have to use a homogenizer to ensure proper tissue lysis in the assay buffer. Any type of homogenizer that you prefer can be used, and as shown on the datasheet, we recommend deproteinizing sample isolation preferably with PCA rather than ass...

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    Hello, I am interested in the product ab83355 ATP assay kit, but I am wondering if it would be suitable for plant root ATP assay. If it is not, which product of your company can I choose? Looking forward to your reply!

    Thank you for contacting us.

    We have optimized ab83355’s assay protocol for samples of mammalian origin. However, theoretically this assay should work with samples from multiple origins including plants. Since we have not done it in house,...

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    Hello,



    I am interested in this product but I am wondering if it would be able to read on 562nm-595nm filters (in your Data sheet it says 570nm).



    I am looking forward to hear from you,

    Thank you for contacting us. Yes, your 562-595 nm filters should work fine for this kit.
    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Dear sir

    tank you , i complete the form and attached,please see the attachment, i hope you can solve my problem as soon as possible.



    with best regards

    Thank you for your response.
    I understand that the standard curve was fine so the kit seems to be working fine. It may well be that the samples have very low level of ATP.
    Your samples are PRP (plasma rich platelet); please bear in mind that pla...

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    Dear sir

    I want to use ATP assay kit colorimetric/fluorometric (Ab83355) for measuring the ATP level in platelet during storage.I have two question for ths assay in platelet:

    1. I dont have liquid N2 or dry ice , if i want to store p...

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    Thank you for your reply. I am sorry it has taken some time to obtain this information for you from our source and I appreciate your patience.

    1. We would not recommend storing the platelet lysate at -70°C since the ATP is very labile and would...

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    Assay worked but no changes between control group and cyanide treatment groups.

    I have contacted the lab regarding your request. From what I can see from your results, the assay did work although there was no significant difference between the cyanide and control group.

    To be able to give you the best possible help, I would...

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    Thank you very much for your quick answer!
    I could find that the concentration of ATP into human plasma is around 1nM! This means that we have to measure a quantity of around 5pg of ATP by wells ! And this is very low!!
    Would you think that your...

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    This kit has been tested using both fluorometric and colourimetric methods and can detect as low as 1 µM of ATP in various samples. Therefore, it is highly unlikely that this kit will be able to detect ATP in your samples as 1 nM is outside of its d...

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    Inquiry: I am looking for a method for analysing the ATP content in blood samples. Could this ATP colorimetric test kit be used for this purpose. If so, could you explain how the sample should be prepared and what volume I would require.

    Thank you for contacting us.

    The source of the kit confirmed that the kit ab83355 is compatible with plasma or serum. In such a case, it is recommended to deproteinise the sample as suggested in ab83355 kit protocol (http://www.abcam.com/ps/produ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"