Anti-HIF2 alpha antibody [ep190b] (ab8365)

Overview

• Product nameAnti-HIF2 alpha antibody [ep190b]See all HIF2 alpha primary antibodies ...
• Description
  Mouse monoclonal [ep190b] to HIF2 alpha
• SpecificityThis antibody is specific for HIF-2 alpha and does not cross-react with HIF-1 alpha.
• Tested applicationsFlow Cyt, ELISA, IHC-Fr, IHC-P, WB more details
• Species reactivity
  Reacts with: Rat, Human
  
• Immunogen

  Human EPAS-1 (HIF2A) amino acids 535-631 fusion protein with proprietary tag.

• Positive controlHypoxic A549 lysate.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferAscitic fluid with 0.1% sodium azide
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Monoclonal
• Clone numberep190b
• MyelomaNS1
• IsotypeIgG1
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Hypoxia

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Nucleotide metabolism
  • Molecular processes
  • Mitochondrial transcription

  • Cancer
  • Cancer Metabolism
  • Response to hypoxia

  • Cancer
  • Invasion/microenvironment
  • Hypoxia
  • HIF

  • Cardiovascular
  • Hypoxia
  • HIF

  • Epigenetics and Nuclear Signaling
  • Cardiovascular/Immune
  • Hypoxia
  • HIF

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • HLH / Leucine Zipper
  • HLH

Applications

Target

• FunctionTranscription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD.
• Tissue specificityExpressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells.
• Involvement in diseaseDefects in EPAS1 are the cause of erythrocytosis familial type 4 (ECYT4) [MIM:611783]. ECYT4 is an autosomal dominant disorder characterized by increased serum red blood cell mass, elevated hemoglobin concentration and hematocrit, and normal platelet and leukocyte counts.
• Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
  Contains 1 PAC (PAS-associated C-terminal) domain.
  Contains 2 PAS (PER-ARNT-SIM) domains.
• Post-translational
  modificationsIn normoxia, is probably hydroxylated on Pro-405 and Pro-531 by EGLN1/PHD1, EGLN2/PHD2 and/or EGLN3/PHD3. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization.
  In normoxia, is hydroxylated on Asn-847 by HIF1AN thus probably abrogating interaction with CREBBP and EP300 and preventing transcriptional activation.
  Phosphorylated on multiple sites in the CTAD.
  The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains.
• Cellular localizationNucleus.

Target information above from: UniProt accession Q99814 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 2034 Human
  • Entrez Gene: 29452 Rat
  • Omim: 603349 Human
  • SwissProt: Q99814 Human
  • SwissProt: Q9JHS1 Rat
  • Unigene: 468410 Human
  • Unigene: 55138 Rat

• Alternative names
  Basic helix loop helix PAS protein MOP2 antibodyBasic-helix-loop-helix-PAS protein MOP2 antibodybHLHe73 antibody

  Class E basic helix-loop-helix protein 73 antibodyECYT4 antibodyEndothelial PAS domain containing protein 1 antibodyEndothelial pas domain protein 1 antibodyEndothelial PAS domain-containing protein 1 antibodyEPAS 1 antibodyEPAS-1 antibodyEPAS1 antibodyEPAS1_HUMAN antibodyHIF 1 alpha like factor antibodyHIF 2 alpha antibodyHIF-1-alpha-like factor antibodyHIF-2-alpha antibodyHIF1 alpha like factor antibodyHIF2-alpha antibodyHIF2A antibodyHLF antibodyHypoxia inducible factor 2 alpha antibodyHypoxia inducible factor 2 alpha subunit antibodyHypoxia-inducible factor 2-alpha antibodyMember of PAS protein 2 antibodyMember of pas superfamily 2 antibodyMOP 2 antibodyMOP2 antibodyPAS domain-containing protein 2 antibodyPASD2 antibody

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Anti-HIF2 alpha antibody [ep190b] images

• 

  Western blot - HIF2 alpha antibody [ep190b] (ab8365)
  
  
  Predicted band size : 100 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HIF2 alpha antibody [ep190b] (ab8365)

  ab8365 at 1/300 dilution, staining HIF2 alpha in cardiomyocytes of human heart tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).
  

• 

  Flow Cytometry - Anti-HIF2 alpha antibody [ep190b] (ab8365)
  Overlay histogram showing HepG2 cells stained with ab8365 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8365, 2µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.
  
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF2 alpha antibody [ep190b] (ab8365)Image from Xiang L et al., Diagn Pathol. 2012 Mar 27;7:32. Fig 5.; doi:10.1186/1746-1596-7-32; 27 March 2012, Diagnostic Pathology 2012, 7:32
  Immunohistochemical analysis of Human breast cancer tissue, staining HIF2 alpha with ab8365.
  
  Antigen retrieval was performed using EDTA buffer in a pressure cooker, before being blocked with normal serum. Samples were incubated with primary antibody (1/2000) overnight at 4°C. Staining was detected using DAB.

Protocols

• Western blot protocol for HIF-2 alpha
• Immunohistochemistry for HIF2 alpha