IL-17 Human ELISA Kit (ab83688)
- Product nameIL-17 Human ELISA KitSee all IL17 kits ...
Intra-assay Sample n Mean SD CV% A 3 49pg/ml 3.8 7.7% B 3 36pg/ml 2.3 6.4% C 3 31pg/ml 0.6 1.9% Inter-assay Sample n Mean SD CV% A 8 50pg/ml 7.8 14.5% B 8 37pg/ml 3 8.2% C 8 31pg/ml 2.1 6.6%
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 3 pg/ml
- Range3.12 pg/ml - 100 pg/ml
Sample specific recovery Sample type Average % Range Serum 121 113% - 130%
- Assay time3h 45m
- Species reactivityReacts with: Human
- Product overview
The IL-17A ELISA is to be used for the in-vitro quantitative determination of IL-17A in supernatant, buffered solutions, or serum and plasma samples. The assay will recognize natural IL-17A. This kit has been configured for research use only and is not to be used in diagnostic procedures.
PRINCIPLE OF THE METHOD
The IL-17A is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for IL-17A has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-17A concentrations and unknowns are pipetted into these wells.
During the first incubation, IL-17A antigen is added to wells. After washing, a biotinylated monoclonal antibody specific for
IL-17A is incubated. Then the enzyme (streptavidin-peroxydase) is added. After incubation and washing to remove all unbound enzyme, a substrate solution which acts on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-17A present in the samples.
The assay recognizes natural and recombinant human IL-17A.
RANGE: 3.12 pg/ml - 100 pg/ml
SENSITIVITY: < 3 pg/ml
To define specificity of this ELISA, several proteins were tested for cross reactivity. There was no cross reactivity observed for any protein tested (IL-1b, IL-6, IL-23, IFNg, IL-17B, IL-17D, IL-17E, IL-17F).
The minimum detectable dose of IL-17A is less than 3 pg/ml. This has been determined by adding 2 standard deviations to the mean optical density obtained when the zero standard was assayed 35 times.
- For research use only.
- Handling of reagents, serum or plasma specimens should be in accordance with local safety procedures , e.g.
CDC/NIH Health manual : " Biosafety in Microbiological and Biomedical Laboratories" 1984.
- Avoid any skin contact with H2SO4 and TMB. In case of contact, wash thoroughly with water.
- Do not eat, drink, smoke or apply cosmetics where kit reagents are used.
- Do not pipette by mouth.
- The stop solution in this kit contains 9.8% sulphuric acid.
- Tested applicationsELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 8 strips 12 units Biotinylated anti IL-17A 1 x 0.4ml Biotinylated Antibody Diluent 1 x 7ml Chromogen TMB 1 x 11ml H2SO4:Stop Reagent 1 x 11ml HRP Diluent 1 x 23ml Standard Diluent: buffer 10X concentrated dilute in distilled water 1 x 25ml Standard: 100 pg/ml Reconstitute with volume of Standard diluent indicated on the vial 2 vials Streptavidin-HRP Add 0.5ml of HRP-Diluent before further dilutions 2 x 5µl Washing Buffer 200Xconcentrated. Dilute in distilled Water 1 x 10ml
- Research Areas
modificationsFound both in glycosylated and nonglycosylated forms.
- CTLA 8CTLA-8CTLA8
- Cytotoxic T lymphocyte associated antigen 8Cytotoxic T lymphocyte associated protein 8Cytotoxic T lymphocyte associated serine esterase 8Cytotoxic T-lymphocyte-associated antigen 8IL 17IL 17AIL-17IL-17AIL17_HUMANIl17aInterleukin 17AInterleukin-17AInterleukin17Interleukin17A
Our Abpromise guarantee covers the use of ab83688 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for IL-17 Human ELISA Kit (ab83688)
This product has been referenced in:
- Yu X et al. Intensive Cytokine induction in Pandemic H1N1 Influenza Virus Infection Accompanied by Robust Production of IL-10 and IL-6. PLoS One 6:e28680 (2011). ELISA . Read more (PubMed: 22174866) »