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Overview

  • Product nameBAFF protein (Active)See all BAFF proteins and peptides ...
  • Protein descriptionRecombinant full length Human BAFF, amino acids 134-285 (Swiss-Prot Q9Y275).

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Biological activityThe ED50 of ab83938 is typically 5-10 ng/ml as measured by its ability to neutralise dexamethasone toxicity using the RPMI 8226 cell line.
  • FormLyophilised:It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, with longer-term storage in aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
  • Storage instructionsStore at +4°C.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferPreservative: None
    Constituents: 10% Trehalose, 1% Human serum albumin
  • Concentration information loading...
  • Sequence notesTheoretical sequence: AVQGPEETVTQDCLQLIADSETPTIQKGS YTFVPWLLSFKRGSALEEK ENKILVKETGYFFIYGQVL YTDKTYAMGHLIQRKKVHVFGDELSLVTL FRCIQNMPE TLPNNSCYSAGIAKLEEGDELQLAIPRENAQISLDGDVT FFGALKLL
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab83938 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
  • Application notesSDS-PAGE: Use at an assay dependent dilution.

    ab83938 migrates as a band between 15 and 20 kDa in SDS-PAGE. This compares with the predicted molecular mass of 17 kDa.

    Due to post-translational modifications ab83938 separates into a number of glycoforms with a pI between 4.7 and 5.5 on 2D PAGE. This is in agreement with the unmodified BAFF that has a predicted pI of 4.9.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        ApoL related ligand TALL 1B cell Activating FactorB lymphocyte stimulator
        B-cell-activating factorBAFFBLYSCD 257CD257CD257 antigendelta BAFFDendritic cell derived TNF like moleculeDendritic cell-derived TNF-like moleculeDTLDTL precursorPRO738soluble formTALL 1TALL-1TALL1THANKTN13B_HUMANTNF and APOL related leukocyte expressed ligand 1TNF homolog that activates apoptosisTNF homolog that activates apoptosis NKFB and JNK.TNF- and APOL-related leukocyte expressed ligand 1TNFSF13BTNFSF20Tumor necrosis factor ligand superfamily member 13BTumor necrosis factor ligand superfamily member 20Tumor necrosis factor like protein ZTNF4Tumor necrosis factor superfamily member 13BUNQ401ZTNF4
      see all
  • FunctionCytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B-and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.
  • Tissue specificityAbundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas.
  • Sequence similaritiesBelongs to the tumor necrosis factor family.
  • Post-translational
    modifications    The soluble form derives from the membrane form by proteolytic processing.
    N-glycosylated.
  • Cellular localizationSecreted and Cell membrane.

    • Target information above from: UniProt accession Q9Y275 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    BAFF protein (Active) images

    • Functional Studies - BAFF protein (ab83938)
      Functional Studies - BAFF protein (ab83938)
      Post-translational modifications result in protein heterogeneity. The densitometry scan demonstrates the purified human cell expressed protein exists in multiple glycoforms, which differ according to their level of post-translational modification.

      The triangle indicates theoretical pI and MW of the protein.

    References for BAFF protein (Active) (ab83938)

    ab83938 has not yet been referenced specifically in any publications.

    Publishing research using ab83938 ? Please let us know so that we can cite the reference in this datasheet

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"