Anti-PCSK9 antibody [mAbcam84041] (ab84041)

Overview

• Product nameAnti-PCSK9 antibody [mAbcam84041]See all PCSK9 primary antibodies ...
• Description
  Mouse monoclonal [mAbcam84041] to PCSK9
• Tested applicationsWB, ICC/IF more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Non Human Primates
• Immunogen

  Recombinant fragment derived from within residues 100 - 200 of Human PCSK9.

  Read Abcam's proprietary immunogen policy

• Positive controlThis antibody gave a positive signal in human heart and human kidney tissue lysates.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferpH: 7.40
  Preservative: 0.02% Sodium azide
  Constituent: PBS
• Concentration information loading...
• PurityConcentrated Culture Supernatant
• Clonality Monoclonal
• Clone numbermAbcam84041
• MyelomaSp2/0-Ag14
• IsotypeIgM
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Cholesterol Metabolism

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Amino acid metabolism

  • Stem Cells
  • Neural Stem Cells
  • Intracellular

  • Signal Transduction
  • Metabolism
  • Lipid metabolism

  • Signal Transduction
  • Metabolism
  • Amino Acids

  • Cardiovascular
  • Lipids / Lipoproteins
  • Lipid Metabolism
  • Cholesterol Metabolism

  • Cell Biology
  • Proteolysis / Ubiquitin
  • Proteolytic enzymes
  • Serine protease
  • Proprotein Convertases

Applications

Target

• FunctionMay be implicated in the differentiation of cortical neurons and may play a role in cholesterol homeostasis.
• Tissue specificityExpressed in neuro-epithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells.
• Involvement in diseaseDefects in PCSK9 are the cause of familial hypercholesterolemia 3 (FH3) [MIM:603776]. FH3 inheritance is autosomal dominant.
• Sequence similaritiesBelongs to the peptidase S8 family.
  Contains 1 peptidase S8 domain.
• Post-translational
  modificationsThe soluble zymogen undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted enzyme.
• Cellular localizationSecreted.

Target information above from: UniProt accession Q8NBP7 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 255738 Human
  • Omim: 607786 Human
  • SwissProt: Q8NBP7 Human
  • Unigene: 18844 Human

• Alternative names
  Convertase subtilisin/kexin type 9 preproprotein antibodyFH3 antibodyHCHOLA3 antibody

  Hypercholesterolemia autosomal dominant 3 antibodyLDLCQ1 antibodyNARC 1 antibodyNARC-1 antibodyNARC1 antibodyNeural apoptosis regulated convertase 1 antibodyNeural apoptosis-regulated convertase 1 antibodyPC 9 antibodyPC9 antibodyPCSK 9 antibodyPCSK9 antibodyPCSK9_HUMAN antibodyProprotein convertase 9 antibodyProprotein convertase PC9 antibodyProprotein convertase subtilisin/kexin type 9 antibodyPSEC0052 antibodySubtilisin/kexin like protease PC9 antibodySubtilisin/kexin-like protease PC9 antibody

  see all

Anti-PCSK9 antibody [mAbcam84041] images

• 

  Western blot - PCSK9 antibody (ab84041)
  All lanes : Anti-PCSK9 antibody [mAbcam84041] (ab84041) at 1/500 dilution
  
  Lane 1 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)
  Lane 2 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  Goat polyclonal Secondary Antibody to Mouse IgM (HRP) at 1/3000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 74 kDa
  Observed band size : 74 kDa
  Additional bands at : 30 kDa,40 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 1 minute
• 

  Immunocytochemistry/ Immunofluorescence - PCSK9 antibody [mAbcam84041] (ab84041)
  ICC/IF image of ab84041 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84041, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2 and MCF7 cells at 5µg/ml.