c-Myc protein (Human) (ab84132)
Constituents: 20% Glycerol, 20mM Tris HCl, 100mM Potassium chloride, 1mM DTT, 0.2mM EDTA, pH 8.0
Our Abpromise guarantee covers the use of ab84132 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: Use at an assay dependent concentration. Ab84132 can be used as a WB positive control in conjunction with ab56.|
|SDS-PAGE||SDS-PAGE: Use at an assay dependent dilution.|
|Functional Studies||FuncS: Use at an assay dependent concentration. Protein-protein interaction assays: 100 Units DNA-protein interaction assays: 100 Units 1 unit equals 1 nanogram of purified protein.|
- AU016757Avian myelocytomatosis viral oncogene homologbHLHe39
- c MycCellular myelocytomatosis oncogeneClass E basic helix-loop-helix protein 39MGC105490MRTLMycMyc proteinMyc proto oncogene proteinMyc proto-oncogene proteinMyc-related translation/localization regulatory factorMYC_HUMANMyc2mycaMyelocytomatosis oncogeneMyelocytomatosis oncogene aNiardNirdOncogene MycOTTHUMP00000158589OTTHUMP00000227763Proto-oncogene c-MycProtooncogene homologous to myelocytomatosis virusRNCMYCTranscription factor p64Transcriptional regulator Myc-AV-Myc avian myelocytomatosis viral oncogene homologv-myc myelocytomatosis viral oncogene homolog (avian)zc-myc
Note=A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
Defects in MYC are a cause of Burkitt lymphoma (BL) [MIM:113970]. A form of undifferentiated malignant lymphoma commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. Note=Chromosomal aberrations involving MYC are usually found in Burkitt lymphoma. Translocations t(8;14), t(8;22) or t(2;8) which juxtapose MYC to one of the heavy or light chain immunoglobulin gene loci.
modificationsPhosphorylated by PRKDC. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome.
Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex.
c-Myc protein (Human) images
Anti-c-Myc antibody [9E11] - ChIP Grade (ab56) at 1/500 dilution +
c-Myc protein (Human) (ab84132) at 0.01 µg
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 4 minutes
References for c-Myc protein (Human) (ab84132)
This product has been referenced in:
- Tremblay R et al. The development of a high-yield recombinant protein bioreactor through RNAi induced knockdown of ATP/ADP transporter in Solanum tuberosum. J Biotechnol 156:59-66 (2011). Read more (PubMed: 21864587) »