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Overview

  • Product namec-Myc protein (Human)See all c-Myc proteins and peptides ...
  • Protein descriptionRecombinant full-length c-Myc expressed in E.Coli, amino acids 1-439, predicted MW 49 kDa, Swiss-Prot P01106. The c-Myc protein has a His-tag and is purified by nickel affinity column.
  • Expression hostE. coli

    • Properties

  • Purification notesab84132 was purified by affinity and FPLC chromatography. The purified recombinant protein is greater than 75% homogeneous based on SDS-PAGE analysis.
  • Biological activityThe c-Myc protein has a His-tag and is purified by nickel affinity column.
  • FormLiquid
  • Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 20% Glycerol, 20mM Tris HCl, 100mM Potassium chloride, 1mM DTT, 0.2mM EDTA, pH 8.0
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab84132 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB WB: Use at an assay dependent concentration. Ab84132 can be used as a WB positive control in conjunction with ab56.
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.
    Functional Studies FuncS: Use at an assay dependent concentration. Protein-protein interaction assays: 100 Units DNA-protein interaction assays: 100 Units 1 unit equals 1 nanogram of purified protein.

    Protein info

    • Alternative names
        AU016757Avian myelocytomatosis viral oncogene homologbHLHe39
        c MycCellular myelocytomatosis oncogeneClass E basic helix-loop-helix protein 39MGC105490MRTLMycMyc proteinMyc proto oncogene proteinMyc proto-oncogene proteinMyc-related translation/localization regulatory factorMYC_HUMANMyc2mycaMyelocytomatosis oncogeneMyelocytomatosis oncogene aNiardNirdOncogene MycOTTHUMP00000158589OTTHUMP00000227763Proto-oncogene c-MycProtooncogene homologous to myelocytomatosis virusRNCMYCTranscription factor p64Transcriptional regulator Myc-AV-Myc avian myelocytomatosis viral oncogene homologv-myc myelocytomatosis viral oncogene homolog (avian)zc-myc
      see all
  • FunctionParticipates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
  • Involvement in diseaseNote=Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors.
    Note=A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
    Defects in MYC are a cause of Burkitt lymphoma (BL) [MIM:113970]. A form of undifferentiated malignant lymphoma commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. Note=Chromosomal aberrations involving MYC are usually found in Burkitt lymphoma. Translocations t(8;14), t(8;22) or t(2;8) which juxtapose MYC to one of the heavy or light chain immunoglobulin gene loci.
  • Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
  • Post-translational
    modifications    Phosphorylated by PRKDC. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome.
    Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex.
  • Cellular localizationNucleus > nucleoplasm. Nucleus > nucleolus.

    • Target information above from: UniProt accession P01106 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    c-Myc protein (Human) images

    • Western blot
      Western blot
      Anti-c-Myc antibody [9E11] - ChIP Grade (ab56) at 1/500 dilution + c-Myc protein (Human) (ab84132) at 0.01 µg

      Secondary
      Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 4 minutes

    References for c-Myc protein (Human) (ab84132)

    This product has been referenced in:
    • Tremblay R  et al. The development of a high-yield recombinant protein bioreactor through RNAi induced knockdown of ATP/ADP transporter in Solanum tuberosum. J Biotechnol 156:59-66 (2011). Read more (PubMed: 21864587) »

    See 1 Publication for this product

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    Western blot Abreview for c-Myc protein (Human)

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    Application Western blot

    Review text: Used us a positive control in western blot among ChIP samples.
    Sample: Human Cell lysate - nuclear (colon)
    Loading amount: 0.01 µg
    Specification: colon
    Gel Running Conditions: Reduced Denaturing (4-12%)
    Blocking step: Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 1&##181;g/mL · Temperature: 4°C

    Other product details
    Dilution: 1/1000
    Incubation time: 16 hour(s) and 0 minute(s) · Temperature: 4°C

    Primary antibody (in addition to 'c-Myc protein (Human)')
    Dilution: 1/1000

    Secondary antibody
    Name: Abcam antibody:(not specified yet)
    Dilution: 1/1000

    Detection
    Detection method: ECL substrate
    Exposure: 1 minute(s) and 0 second(s)
    Bands: Specific: around 55 kDa
    Negative control: BSA ChIPed cell lysate nuclear
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    Submitted Aug 26 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"