Anti-PRMT4 antibody (ab84370)

Overview

• Product nameAnti-PRMT4 antibodySee all PRMT4 primary antibodies ...
• Description
  Rabbit polyclonal to PRMT4
• Tested applicationsIHC-P, WB more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide, corresponding to a region within C terminal amino acids 550-585 of Human PRMT4 (SwissProt Q86X55).

• Positive controlHuman breast adenocarcinoma. Human prostate adenocarcinoma.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: 0.1% BSA, Tris buffered saline
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesAntibody was affinity purified using an epitope specific to PRMT4 immobilized on solid support.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Lipid metabolism

  • Metabolism
  • Pathways and Processes
  • Cofactors, Vitamins / minerals
  • Co-factors

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Metabolism of lipids and lipoproteins

  • Cancer
  • Oncoproteins/suppressors
  • Tumor suppressors
  • p53 pathway

  • Signal Transduction
  • Signaling Pathway
  • Nuclear Signaling
  • Nuclear Hormone Receptors
  • Co-activators/co-repressors

  • Epigenetics and Nuclear Signaling
  • Chromatin Modifying Enzymes
  • Methylation
  • Arginine Methylation

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Other factors

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Co-factors

  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Cell Cycle Inhibitors
  • p53

  • Cell Biology
  • Cell Cycle
  • Cell Cycle Inhibitors
  • p53

Applications

Target

• FunctionMethylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activate transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg-2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs.
• Tissue specificityOverexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia.
• Sequence similaritiesBelongs to the protein arginine N-methyltransferase family.
• Post-translational
  modificationsAuto-methylated on Arg-550. Methylation enhances transcription coactivator activity. Methylation is required for its role in the regulation of pre-mRNA alternative splicing.
  Phosphorylation at Ser-216 interferes with S-adenosyl-L-methionine binding and strongly reduces methyltransferase activity (By similarity). Phosphorylation at Ser-216 is strongly increased during mitosis, and decreases rapidly to a very low, basal level after entry into the G1 phase of the cell cycle. Phosphorylation at Ser-216 may promote location in the cytosol.
• Cellular localizationNucleus. Cytoplasm. Mainly nuclear during the G1, S and G2 phases of the cell cycle. Cytoplasmic during mitosis, after breakup of the nuclear membrane.

Target information above from: UniProt accession Q86X55 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 10498 Human
  • Entrez Gene: 10498 Human
  • Omim: 603934 Human
  • SwissProt: Q86X55 Human
  • SwissProt: Q86X55 Human
  • Unigene: 323213 Human

• Alternative names
  CARM1 antibodyCARM1_HUMAN antibodyCoactivator associated arginine methyltransferase 1 antibody

  Coactivator-associated arginine methyltransferase 1 antibodyHistone arginine methyltransferase CARM 1 antibodyHistone arginine methyltransferase CARM1 antibodyHistone-arginine methyltransferase CARM1 antibodyPRMT 4 antibodyPRMT4 antibodyProtein arginine methyltransferase antibodyProtein arginine N methyltransferase 4 antibodyProtein arginine N-methyltransferase 4 antibody

  see all

Anti-PRMT4 antibody images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PRMT4 antibody (ab84370)
  ab84370 at 1/100, staining human PRMT4 in a section of human breast adenocarcinoma by immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PRMT4 antibody (ab84370)
  ab84370 at 1/500, staining human PRMT4 in a section of human prostate adenocarcinoma by immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).