Anti-GPSM2 antibody (ab84571)

Overview

• Product nameAnti-GPSM2 antibodySee all GPSM2 primary antibodies ...
• Description
  Rabbit polyclonal to GPSM2
• Tested applicationsICC/IF, IHC-Fr, WB, IHC-P more details
• Species reactivity
  Reacts with: Mouse, Human
  Predicted to work with: Rat, Chicken, Cow, Dog, Zebrafish
• Immunogen

  Synthetic peptide corresponding to a region within N terminal amino acids 72-121 (YFYLHDYAKA LEYHHHDLTL ARTIGDQLGE AKASGNLGNT LKVLGNFDEA) of Human GPSM2 (NP_037428).

  Run BLAST with Run BLAST with
• Positive controlHuman fetal liver lysate.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: 2% Sucrose, PBS
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Signaling Pathway
  • G Protein Signaling
  • Heterotrimeric G Proteins
  • Regulators

  • Cell Biology
  • Cell Cycle
  • Cell Division
  • Spindle

Applications

Target

• FunctionPlays an important role in spindle pole orientation. Interacts and contributes to the functional activity of G(i) alpha proteins. Acts to stabilize the apical complex during neuroblast divisions.
• Tissue specificityUbiquitously expressed.
• Involvement in diseaseDefects in GPSM2 are the cause of deafness autosomal recessive type 82 (DFNB82) [MIM:613557]. DFNB82 is a form of non-syndromic deafness characterized by prelingual, bilateral, severe, sensorineural hearing loss. There are no symptoms of vestibular dysfunction.
• Sequence similaritiesBelongs to the GPSM family.
  Contains 4 GoLoco domains.
  Contains 8 TPR repeats.
• Cellular localizationCytoplasm. Cytoplasm > cell cortex. Localizes in the cytoplasm in the interphase and at cell periphery in the metaphase.

Target information above from: UniProt accession P81274 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 29899 Human
  • Entrez Gene: 29899 Human
  • Entrez Gene: 76123 Mouse
  • Entrez Gene: 362021 Rat
  • Omim: 609245 Human
  • SwissProt: P81274 Human
  • SwissProt: P81274 Human
  • SwissProt: Q8VDU0 Mouse

  • Unigene: 584901 Human
  • Unigene: 658489 Human
  • Unigene: 226941 Mouse
  • Unigene: 144235 Rat

  see all

• Alternative names
  DFNB82 antibodyG protein signalling modulator 2 (AGS3 like C. elegans) antibodyG protein signalling modulator 2 antibody

  G-protein-signaling modulator 2 antibodyGpsm2 antibodyGPSM2_HUMAN antibodyHGNC:29501 antibodyLGN antibodyLGN protein antibodyMosaic protein LGN antibodyPins antibody

  see all

Anti-GPSM2 antibody images

• 

  Western blot - GPSM2 antibody (ab84571)
  Anti-GPSM2 antibody (ab84571) at 1 µg/ml (in 5% skim milk / PBS buffer) + human fetal liver lysate at 10 µg
  
  Secondary
  HRP conjugated anti-Rabbit IgG at 1/50000 dilution
  
  Predicted band size : 76 kDa
  Observed band size : 85 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunocytochemistry/ Immunofluorescence - GPSM2 antibody (ab84571)
  ICC/IF image of ab84571 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84571, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPSM2 antibody (ab84571)
  ab84571 at 5 µg/ml staining GPSM2 in Human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPSM2 antibody (ab84571)
  ab84571 at 5 µg/ml staining GPSM2 in Human placenta tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).