Anti-BMP7 antibody (ab84684)
Key features and details
- Rabbit polyclonal to BMP7
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-BMP7 antibody
See all BMP7 primary antibodies -
Description
Rabbit polyclonal to BMP7 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Horse, Chicken, Pig -
Immunogen
Synthetic peptide corresponding to Human BMP7 aa 300-400 conjugated to keyhole limpet haemocyanin.
(Peptide available asab104212) -
Positive control
- Recombinant Human BMP7 protein (ab87055) can be used as a positive control in WB. This antibody gave a positive signal in both Human and Rat Brain Tissue Lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab84684 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
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IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa). |
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis. -
Tissue specificity
Expressed in the kidney and bladder. Lower levels seen in the brain. -
Sequence similarities
Belongs to the TGF-beta family. -
Developmental stage
Expressed in the developing eye, brain and ear during embryogenesis. -
Post-translational
modificationsSeveral N-termini starting at positions 293, 300, 315 and 316 have been identified by direct sequencing resulting in secretion of different mature forms (PubMed:17977014). -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 395494 Chicken
- Entrez Gene: 655 Human
- Entrez Gene: 12162 Mouse
- Entrez Gene: 492315 Pig
- Entrez Gene: 85272 Rat
- Omim: 112267 Human
- SwissProt: P18075 Human
- SwissProt: P23359 Mouse
see all -
Alternative names
- BMP 7 antibody
- BMP-7 antibody
- Bmp7 antibody
see all
Images
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All lanes : Anti-BMP7 antibody (ab84684) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Brain (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa
Additional bands at: 10 kDa, 33 kDa, 38 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds -
IHC image of BMP7 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab84684, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Anti-BMP7 antibody (ab84684) at 1 µg/ml + Brain (Rat) Tissue Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 49 kDa
Additional bands at: 37 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab84684 has been referenced in 5 publications.
- Niikura T et al. Immunohistochemical analysis revealed the expression of bone morphogenetic proteins-4, 6, 7, and 9 in human induced membrane samples treated with the Masquelet technique. J Orthop Surg Res 17:29 (2022). PubMed: 35033126
- Kim S et al. Newly designed Protein Transduction Domain (PTD)-mediated BMP-7 is a potential therapeutic for peritoneal fibrosis. J Cell Mol Med 24:13507-13522 (2020). PubMed: 33079436
- Kim S et al. Micellized Protein Transduction Domain-Bone Morphogenetic Protein-7 Efficiently Blocks Renal Fibrosis Via Inhibition of Transforming Growth Factor-Beta-Mediated Epithelial-Mesenchymal Transition. Front Pharmacol 11:591275 (2020). PubMed: 33364962
- Li C et al. Neural EGFL like 1 as a potential pro-chondrogenic, anti-inflammatory dual-functional disease-modifying osteoarthritis drug. Biomaterials 226:119541 (2020). PubMed: 31634652
- Sun N et al. Monocrotaline pyrrole enhanced bone morphogenetic protein 7 signaling transduced by alternative activin A receptor type 2A in pulmonary arterial smooth muscle cells. Eur J Pharmacol 863:172679 (2019). PubMed: 31542483