Anti-XPB antibody (ab85408)

Overview

• Product nameAnti-XPB antibodySee all XPB primary antibodies ...
• Description
  Rabbit polyclonal to XPB
• Tested applicationsIHC-P more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide corresponding to a region between residue 575 and 625 of Human XPB (NP_000113.1).

• Positive controlHuman Anaplastic Thyroid Carcinoma, Bladder Cell Carcinoma, Breast Carcinoma, Pancreatic Islet Cell Tumor and Prostate Carcinoma.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: 0.1% BSA, Tris buffered saline
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Nucleotide metabolism
  • Purine and pyrimidine synthesis

  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Hypoxia

  • Metabolism
  • Types of disease
  • Cancer

  • Cancer
  • Cancer Metabolism
  • Response to hypoxia

  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Damage & Repair
  • Nucl. Excision Repair

Applications

Target

• FunctionATP-dependent 3'-5' DNA helicase, component of the core-TFIIH basal transcription factor, involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. Acts by opening DNA either around the RNA transcription start site or the DNA damage.
• Involvement in diseaseDefects in ERCC3 are the cause of xeroderma pigmentosum complementation group B (XP-B) [MIM:610651]; also known as xeroderma pigmentosum II (XP2) or XP group B (XPB) or xeroderma pigmentosum group B combined with Cockayne syndrome (XP-B/CS). Xeroderma pigmentosum is an autosomal recessive pigmentary skin disorder characterized by solar hypersensitivity of the skin, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. Some XP-B patients present features of Cockayne syndrome, including dwarfism, sensorineural deafness, microcephaly, mental retardation, pigmentary retinopathy, ataxia, decreased nerve conduction velocities.
  Defects in ERCC3 are a cause of trichothiodystrophy photosensitive (TTDP) [MIM:601675]. TTDP is an autosomal recessive disease characterized by sulfur-deficient brittle hair and nails, ichthyosis, mental retardation, impaired sexual development, abnormal facies and cutaneous photosensitivity correlated with a nucleotide excision repair (NER) defect. Neonates with trichothiodystrophy and ichthyosis are usually born with a collodion membrane. The severity of the ichthyosis after the membrane is shed is variable, ranging from a mild to severe lamellar ichthyotic phenotype. There are no reports of skin cancer associated with TTDP.
• Sequence similaritiesBelongs to the helicase family. RAD25/XPB subfamily.
  Contains 1 helicase ATP-binding domain.
  Contains 1 helicase C-terminal domain.
• Cellular localizationNucleus.

Target information above from: UniProt accession P19447 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 2071 Human
  • Omim: 133510 Human
  • SwissProt: P19447 Human
  • Unigene: 469872 Human

• Alternative names
  Basic transcription factor 2 89 kDa subunit antibodyBTF 2 antibodyBTF2 antibody

  BTF2 p89 antibodyDNA excision repair protein ERCC-3 antibodyDNA repair protein complementing XP-B cells antibodyERCC 3 antibodyERCC3 antibodyERCC3_HUMAN antibodyExcision Repair Cross-complementing Rodent Repair deficiency complementation Group 3 antibodyGTF2H antibodyRAD 25 antibodyRAD25 antibodyTFIIH 89 kDa subunit antibodyTFIIH antibodyTFIIH basal transcription factor complex 89 kDa subunit antibodyTFIIH basal transcription factor complex helicase XPB subunit antibodyTFIIH p89 antibodyXeroderma pigmentosum group B-complementing protein antibody

  see all

Anti-XPB antibody images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - XPB antibody (ab85408)
  ab85408, at 1/250 dilution, staining XPB in formalin-fixed, paraffin-embedded human bladder cell carcinoma by Immunohistochemistry using DAB staining.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - XPB antibody (ab85408)
  ab85408, at 1/500 dilution, staining XPB in formalin-fixed, paraffin-embedded human prostate carcinoma by Immunohistochemistry using DAB staining.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPB antibody (ab85408)Image from Yu M et al., PLoS One. 2012;7(5):e34185. Epub 2012 May 16. Fig 2.; doi:10.1371/journal.pone.0034185; May 16, 2012, PLoS ONE 7(5): e34185.
  Immunohistochemical analysis of Human hair follicle bulb tissue, staining XPB with ab85408.
  
  Antigen retrieval was performed by heat mediation in antigen target retrieval solution. Samples were blocked with goat serum followed by incubation with primary antibody overnight at 4°C. An AlexaFluor®594-conjugated anti-rabbit IgG was used as the secondary antibody.