Overview
Product nameSCF protein (Active)See all SCF proteins and peptides ...
Protein descriptionRecombinant fragment, corresponding to amino acids 26-189 of Mouse SCF with His tag produced in the endosperm tissue of barley grain (Hordeum vulgare); 182 amino acids with tag, Predicted MWt 20.6 kDa (UniProt P20826).
Expression hostBarley
Properties
Purity> 95
% by SDS-PAGE
Purification notesSterile filtered through a 0.2 µm filter.
Endotoxin level is less than 0.005ng per µg (0.05EU/µg) as measured by turbidimetric kinetic assay.
ab88278 carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test using Human 10-plex Cytokine Assay measuring IL6, TNF-alpha and IL1beta induction.
Biological activityBioactivity is assayed by measuring the dose dependent effect of Mouse SCF on proliferation of the factor-dependent Human erythroleukemic cell line TF-1. The ED50 lies typically in the range of 8.4-12.8 ng/ml.
FormLyophilised:Reconstitute the lyophilized protein in sterile phosphate-buffered saline to a concentration of no less than 100 µg/ml. For long term storage of the reconstituted solution it is recommended to add a carrier protein (0.1% HSA or BSA).
Storage instructionsThe lyophilized protein is stable for a few weeks at room temperature, but best stored at -20°C.This product is an active protein and may elicit a biological response in vivo, handle with caution.
Storage bufferPreservative: None
Constituents: 0.7M Sodium chloride, 30mM Sodium acetate, pH 4.5
Concentration information loading...
Additional notesReconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab88278
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
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Notes |
| Functional Studies |
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| SDS-PAGE |
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Application notesFuncS: Use at an assay dependent dilution.
SDS-PAGE: Use at an assay dependent dilution. As a result of glycosylation, the recombinant protein migrates as three bands with an apparent molecular mass of 24, 23, 21 kDa in SDS-PAGE.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
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Alternative names
C kit ligandC-kit ligandCkit ligand
DKFZp686F2250familial progressive hyperpigmentation 2FPH2KIT ligandKitlKITLGKL 1KL1Mast cell growth factorMGFSCFSCF_HUMANSFSHEP7sKITLGSoluble KIT ligandSteel factorStem cell factorStem cell factor precursor
see all
FunctionLigand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.
Involvement in diseaseDefects in KITLG are the cause of familial progressive hyperpigmentation (FPH) [MIM:145250]; also called melanosis universalis hereditaria (MUH). FPH is an autosomal-dominantly inherited disorder characterized by hyperpigmented patches in the skin, present in early infancy and increasing in size and number with age.
Sequence similaritiesBelongs to the SCF family.
Developmental stageActs in the early stages of hematopoiesis.
Post-translational
modificationsA soluble form (sKITLG) is produced by proteolytic processing of isoform 1 in the extracellular domain.
Found in two differentially glycosylated forms, LMW-SCF and HMW-SCF. LMW-SCF is fully N-glycosylated at Asn-145, partially N-glycosylated at Asn-90, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97 or Asn-118. HMW-SCF is N-glycosylated at Asn-118, Asn-90 and Asn-145, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97.
A soluble form exists as a cleavage product of the extracellular domain.
Cellular localizationSecreted; Cell membrane and Cell membrane. Cytoplasm > cytoskeleton.
References for SCF protein (Active) (ab88278)
ab88278
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
