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Overview

  • Product nameIGFBP3 protein (Active)See all IGFBP3 proteins and peptides ...
  • Protein descriptionRecombinant full length Human IGFBP3 expressed in modified human 293 cells; amino acids 28-291 (264aa), Predicted MWt 28.7 kDa (Swiss-Prot P17936).
  • Expression hostHEK 293 cells

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Biological activityActivity: The ED50 of ab88368 is typically 0.13-0.20 µg/ml as measured by its ability to neutralize rhIGF-II mediated proliferation of the MCF7 adenocarcinoma cell line.
  • FormLyophilised:It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
  • Storage instructionsShipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferPreservative: None
    Constituents: 10% Trehalose, 1% Human serum albumin
  • Concentration information loading...
  • Sequence notesGASSGGLGPVVRCEPCDARALAQCAPPPAVCAELVREPGC GCCLTC ALSEGQPCGIYTERCGSGLRCQPSPDEARPLQ ALLDGRGLCVNASA VSRLRAYLLPAPPAPGNASESEED RSAGSVESPSVSSTHRVSDPKF HPLHSKIIIIKKGHAK DSQRYKVDYESQSTDTQNFSSESKRETEYG PCRREMED TLNHLKFLNVLSPRGVHIPNCDKKGFYKKKQCRPSKGR KRGFCWCVDKYGQPLPGYTTKGKEDVHCYSMQSK
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab88368 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies
    SDS-PAGE
  • Application notesFuncS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.

    Molecular Mass: ab88368 migrates as a broad band between 40 and 45 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with the unmodified protein that has a predicted molecular mass of 28.7 kDa. ab88368 consists of 25-35% carbohydrate by weight.

    pI: ab88368 separates into a number of isoforms with a pI between 6.8 and 8.2 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified protein that has a predicted pI of 8.76.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        Acid stable subunit of the 140 K IGF complexBinding protein 29Binding protein 53
        BP 53BP53Growth hormone dependent binding proteinIBP 3IBP-3IBP3IBP3_HUMANIGF binding protein 3IGF-binding protein 3IGFBP 3IGFBP-3IGFBP3Insulin Like Growth Factor Binding Protein 3Insulin-like growth factor-binding protein 3
      see all
  • FunctionIGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
  • Tissue specificityExpressed by most tissues. Present in plasma.
  • Sequence similaritiesContains 1 IGFBP N-terminal domain.
    Contains 1 thyroglobulin type-1 domain.
  • Developmental stageIGFBP3 levels are higher during extrauterine life and peak during puberty.
  • DomainThe thyroglobulin type-1 domain mediates interaction with HN.
  • Post-translational
    modifications    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P17936 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    IGFBP3 protein (Active) images

    • Functional Studies - IGFBP3 protein (Active) (ab88368)
      Functional Studies - IGFBP3 protein (Active) (ab88368)
      Densitometry of protein isoforms visualised by 2-DE.
      The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
      The triangle indicates the theoretical MWt and pI of the protein.

    References for IGFBP3 protein (Active) (ab88368)

    ab88368 has not yet been referenced specifically in any publications.

    Publishing research using ab88368 ? Please let us know so that we can cite the reference in this datasheet

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"