Anti-CCRL2 antibody (ab88632)

Overview

• Product nameAnti-CCRL2 antibodySee all CCRL2 primary antibodies ...
• Description
  Mouse polyclonal to CCRL2
• Tested applicationsWB, Flow Cyt more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Full length Human protein (NP_003956)

• Positive controlRAW 264.7 cell lyate, NIH/3T3 cell lysate, transfected 293 cells

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: 1X PBS, pH 7.2
• Concentration information loading...
• PurityProtein A purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Signaling Pathway
  • G Protein Signaling
  • GPCR

  • Microbiology
  • Protein
  • Human Protein
  • Defensin

  • Immunology
  • Innate Immunity
  • Macrophage / Inflamm.

Applications

Target

• FunctionReceptor for CCL19 and chemerin/RARRES2. Does not appear to be a signaling receptor, but may have a role in modulating chemokine-triggered immune responses by capturing and internalizing CCL19 or by presenting RARRES2 ligand to CMKLR1, a functional signaling receptors. Plays a critical role for the development of Th2 responses.
• Tissue specificityExpressed abundantly in immunal tissues such as spleen, fetal liver, lymph node and bone marrow. Strong expression also in lung and heart. Expressed in almost all hematopoietic cells including monocytes, macrophages, PMNs, T-cells (both CD4+ and CD8+), monocyte-derived iDCs, NK cells, and CD34+ progenitor cells. B cells expressed isoform 1 but not isoform 2. Up-regulated on synovial neutrophils of rheumatoid arthritis patients.
• Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
• DomainLacks the conserved DRYLAIV motif in the second intracellular loop that is required for signaling of functional chemokine receptors.
• Cellular localizationCell membrane.

Target information above from: UniProt accession O00421 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 9034 Human
  • Entrez Gene: 9034 Human
  • Entrez Gene: 54199 Mouse
  • Entrez Gene: 316019 Rat
  • Omim: 608379 Human
  • SwissProt: O00421 Human
  • SwissProt: O00421 Human
  • SwissProt: O35457 Mouse

  • Unigene: 535713 Human
  • Unigene: 7336 Mouse
  • Unigene: 67232 Rat

  see all

• Alternative names
  C-C chemokine receptor-like 2 antibodyCCRL2 antibodyCCRL2_HUMAN antibody

  Chemokine (C-C motif) receptor like 2 antibodyChemokine receptor CCR11 antibodyChemokine receptor X antibodyCKRX antibodyCRAM A antibodyCRAM B antibodyCRAMA antibodyCRAMB antibodyFLJ55815 antibodyHCR antibodyMGC116710 antibodyMGC34104 antibodyPutative MCP 1 chemokine receptor antibodyPutative MCP-1 chemokine receptor antibody

  see all

Anti-CCRL2 antibody images

• 

  Flow Cytometry - CCRL2 antibody (ab88632)
  FACS analysis of negative control 293 cells (black) and CCRL2-expressing 293 cells (green) using ab88632.
• 

  Western blot - CCRL2 antibody (ab88632)
  Anti-CCRL2 antibody (ab88632) at 1 µg/ml + Raw 264.7 cell lysate at 50 µg
  
  Predicted band size : 40 kDa
  
• 

  Western blot - CCRL2 antibody (ab88632)
  Anti-CCRL2 antibody (ab88632) at 1 µg/ml + NIH/3T3 cell lysate at 50 µg
  
  Predicted band size : 40 kDa
  
• 

  Western blot - CCRL2 antibody (ab88632)
  All lanes : Anti-CCRL2 antibody (ab88632) at 1 µg/ml
  
  Lane 1 : CCRL2-transfected 293T cell lysate
  Lane 2 : Non-transfected 293T cell lysate
  
  Lysates/proteins at 25 µg per lane.
  
  
  Predicted band size : 40 kDa
  Observed band size : 38 kDa (why is the actual band size different from the predicted?)