Overview
- Product nameAnti-p75 NGF Receptor antibodySee all p75 NGF Receptor primary antibodies ...
- DescriptionRabbit polyclonal to p75 NGF Receptor
- SpecificityThis antibody recognises mouse NGF Receptor
- Tested applicationsICC/IF, Dot Blot, IHC-P, IHC-Fr, IP, WB, IHC-FoFr, ICC, Flow Cyt more details
- Species reactivityReacts with: Mouse, Rat, Human
- Immunogen
An extracellular fragment from the mouse p75 receptor (amino acids 43-161).
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferLiquid antiserum
- PurityWhole antiserum
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab8874 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC/IF | ICC/IF: Use at an assay dependent dilution. |
| Dot Blot | Dot: Use at an assay dependent dilution. |
| IHC-P | IHC-P: Use at an assay dependent dilution. |
| IHC-Fr | IHC-Fr: 1/400. PubMed: 169737171/100. |
| IP | IP: Use at an assay dependent dilution. |
| WB | WB: Use at an assay dependent dilution. |
| IHC-FoFr | IHC-FoFr: 1/100. |
| ICC | ICC: 1/500. |
| Flow Cyt | Flow Cyt: Use at an assay dependent dilution. |
Target
- FunctionLow affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells.
- Sequence similaritiesContains 1 death domain.
Contains 4 TNFR-Cys repeats. - DomainDeath domain is responsible for interaction with RANBP9.
The extracellular domain is responsible for interaction with NTRK1. - Post-translational
modificationsN- and O-glycosylated.
O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.
Phosphorylated on serine residues. - Cellular localizationMembrane.
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Database links
- Entrez Gene: 4804 Human
- Entrez Gene: 4804 Human
- Entrez Gene: 18053 Mouse
- Entrez Gene: 24596 Rat
- Omim: 162010 Human
- SwissProt: P08138 Human
- SwissProt: P08138 Human
- SwissProt: Q9Z0W1 Mouse
- SwissProt: P07174 Rat
- Unigene: 415768 Human
- Unigene: 681726 Human
- Unigene: 283893 Mouse
- Unigene: 10980 Rat
see all
Target information above from: UniProt accession
P08138
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- CD271 antibodyCD271 antigen antibodyGp80 LNGFR antibody
- Gp80 LNGFR antibodyGp80-LNGFR antibodyLow affinity nerve growth factor receptor antibodyLow affinity nerve growth factor receptor antibodyLow affinity neurotrophin receptor p75NTR antibodyLow affinity neurotrophin receptor p75NTR antibodyLow-affinity nerve growth factor receptor antibodyNerve growth factor receptor antibodyNerve growth factor receptor TNFR superfamily member 16 antibodyNGF receptor antibodyNGF receptor antibodyNGFR antibodyp75 ICD antibodyp75 ICD antibodyp75 Neurotrophin receptor antibodyp75 NTR antibodyp75(NTR) antibodyp75NTR antibodyTNFR Superfamily Member 16 antibodyTNFRSF16 antibodyTNR16_HUMAN antibodyTumor necrosis factor receptor superfamily member 16 antibody
see all
Anti-p75 NGF Receptor antibody images
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Flow Cytometry - p75 NGF Receptor antibody (ab8874)NG108-15 cells, a fusion of mouse neuroblastoma and rat glioma cells, were used for a FACS analysis. Cells were incubated for one hour with Ab8874 at a 1:100 dilution, and subsequently with an anti-rabbit IgG conjugated to FITC. A 96% shift was seen relative to control cells treated with secondary antibody only. (note; yellow = control, red = result).
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Immunohistochemistry (PFA perfusion fixed frozen sections) - p75 NGF Receptor antibody (ab8874)This image is courtesy of an anonymous Abreviewab11382 staining normal mouse brain tissue sections by IHC-FoFr. Sections were PFA fixed and blocked with 0.5% TNB for 30 minutes at 25°C. The primary antibody was diluted 1/100 and incubated with the sample for 18 hours at 4°C. An Alexa Fluor® 555 conjugated goat anti-rabbit antibody, diluted 1/500, was used as the secondary. This image (taken at 80x) demonstrates p75 positive neurons in the basal forebrain.
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Western blot - Anti-p75 NGF Receptor antibody (ab8874)Anti-p75 NGF Receptor antibody (ab8874) at 1/500 dilution + NIH-3T3 cells at 10 µg
Secondary
HRP-conjugated Donkey anti-Rabbit polyclonal at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
Exposure time : 12 minutesThis image is courtesy of an anonymous Abreview.
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Western blot - Anti-p75 NGF Receptor antibody (ab8874)Anti-p75 NGF Receptor antibody (ab8874) at 1/500 dilution + whole cell lysate prepared from human WiDr colorectal cancer cell line at 5 µg
Secondary
HRP conjugated donkey anti-rabbit polyclonal at 1/5000 dilution
developed using the ECL technique
Exposure time : 12 minutesImage courtesy of an anonymous Abreview.
References for Anti-p75 NGF Receptor antibody (ab8874)
This product has been referenced in:
- Cekic M et al. Progesterone treatment alters neurotrophin/proneurotrophin balance and receptor expression in rats with traumatic brain injury. Restor Neurol Neurosci 30:115-26 (2012). WB ; Rat . Read more (PubMed: 22232032) »
- Li X et al. Uniaxial mechanical strain modulates the differentiation of neural crest stem cells into smooth muscle lineage on micropatterned surfaces. PLoS One 6:e26029 (2011). IHC-Fr ; Human . Read more (PubMed: 22016804) »
