Anti-EXOSC3 antibody (ab88859)
Key features and details
- Mouse polyclonal to EXOSC3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-EXOSC3 antibody
See all EXOSC3 primary antibodies -
Description
Mouse polyclonal to EXOSC3 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human EXOSC3. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- WB: Human pancreas tissue lysate; EXOSC3 transfected 293T cell lysate ICC/IF: HepG2 cell
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab88859 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
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ICC/IF |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa). |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Relevance
EXOSC3 is a component of the exosome 3'->5' exoribonuclease complex, a complex that degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions. It is required for the 3'processing of the 7S pre-RNA to the mature 5.8S rRNA. Has a 3'-5' exonuclease activity. [Swiss-Prot] -
Cellular localization
Cytoplasm. Nucleus, nucleolus. -
Database links
- Entrez Gene: 51010 Human
- Omim: 606489 Human
- SwissProt: Q9NQT5 Human
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Alternative names
- bA3J10.7 antibody
- CGI 102 antibody
- CGI-102 antibody
see all
Images
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Anti-EXOSC3 antibody (ab88859) at 1 µg/ml + human pancreas tissue lysate at 50 µg
Predicted band size: 30 kDa
Observed band size: 30 kDa -
All lanes : Anti-EXOSC3 antibody (ab88859) at 1 µg/ml
Lane 1 : lysate from EXOSC3 transfected 293T cells
Lane 2 : lysate from non transfected 293T cells
Lysates/proteins at 25 µg per lane.
Predicted band size: 30 kDa
Observed band size: 30 kDa -
ab88859, at 10 µg/ml, staining EXOSC3 in paraformaldehyde fixed, permeabilized HepG2 cell by Immunofluorescence.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab88859 has not yet been referenced specifically in any publications.