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Anti-Acyltransferase like 1 antibody (ab88871)

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Overview

  • Product nameAnti-Acyltransferase like 1 antibodySee all Acyltransferase like 1 primary antibodies ...
  • Description
    Mouse polyclonal to Acyltransferase like 1
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human Acyltransferase like 1 (NP_060309.2, amino acids 1-544)

  • Positive controlHuman pancreas tissue lysate, A431 cell lysate and Acyltransferase like 1 transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab88871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use a concentration of 1 µg/ml. Predicted molecular weight: 60 kDa.

Target

  • FunctionPossesses both acyltransferase and acetyltransferase activities. Activity is calcium-dependent. Involved in platelet-activating factor (PAF) biosynthesis by catalyzing the conversion of the PAF precursor, 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF) into 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (PAF). Also converts lyso-PAF to 1-alkyl-phosphatidylcholine (PC), a major component of cell membranes and a PAF precursor. Under resting conditions, acyltransferase activity is preferred. Upon acute inflammatory stimulus, acetyltransferase activity is enhanced and PAF synthesis increases.
  • PathwayLipid metabolism; phospholipid metabolism.
  • Sequence similaritiesBelongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.
    Contains 2 EF-hand domains.
  • DomainThe HXXXXD motif is essential for acyltransferase activity.
  • Cellular localizationEndoplasmic reticulum membrane. Golgi apparatus membrane.

    • Target information above from: UniProt accession Q7L5N7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        1 acylglycerophosphocholine O acyltransferase antibody1 alkylglycerophosphocholine O acetyltransferase antibody1-acylglycerophosphocholine O-acyltransferase antibody
        1-alkylglycerophosphocholine O-acetyltransferase antibodyA330042H22 antibodyAcetyl CoA:lyso PAF acetyltransferase antibodyAcetyl-CoA:lyso-PAF acetyltransferase antibodyAcetyl-CoA:lyso-platelet-activating factor acetyltransferase antibodyacyl CoA:lysophosphatidylcholine acyltransferase 2 antibodyAcyl-CoA:lysophosphatidylcholine acyltransferase member 1 antibodyAcyltransferase like 1A antibodyAcyltransferase-like 1 antibodyAYTL1 antibodyAytl1a antibodyDKFZp686H22112 antibodyFLJ20481 antibodylpafat1 antibodyLPC acyltransferase 2 antibodyLPCAT-2 antibodyLPCAT2 antibodyLyso PAF acetyltransferase antibodyLyso platelet activating factor (PAF) acetyltransferase antibodyLyso-PAF acetyltransferase antibodyLysoPAFAT antibodyLysoPAFAT/LPCAT2 antibodyLysoPC acyltransferase 2 antibodyLysophosphatidylcholine acyltransferase 2 antibodyOTTMUSP00000029641 antibodyPCAT2_HUMAN antibodyRGD1563994 antibody
      see all

    Anti-Acyltransferase like 1 antibody images

    • Western blot - Acyltransferase like 1 antibody (ab88871)
      Western blot - Acyltransferase like 1 antibody (ab88871)
      Anti-Acyltransferase like 1 antibody (ab88871) at 1 µg/ml + Human pancreas tissue lysate at 50 µg

      Predicted band size : 60 kDa
      Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    • Western blot - Acyltransferase like 1 antibody (ab88871)
      Western blot - Acyltransferase like 1 antibody (ab88871)
      Anti-Acyltransferase like 1 antibody (ab88871) at 1 µg/ml + A431 cell lysate at 50 µg

      Predicted band size : 60 kDa
      Observed band size : 60 kDa
    • Western blot - Acyltransferase like 1 antibody (ab88871)
      Western blot - Acyltransferase like 1 antibody (ab88871)
      All lanes : Anti-Acyltransferase like 1 antibody (ab88871) at 1 µg/ml

      Lane 1 : Acyltransferase like 1 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.


      Predicted band size : 60 kDa
      Observed band size : 50 kDa (why is the actual band size different from the predicted?)

    References for Anti-Acyltransferase like 1 antibody (ab88871)

    ab88871 has not yet been referenced specifically in any publications.

    Publishing research using ab88871 ? Please let us know so that we can cite the reference in this datasheet

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    Western blot abreview for Anti-Acyltransferase like 1 antibody

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    Abreviews
    Application Western blot
    Sample Human Cell lysate - whole cell (HeLa Cells)
    Loading amount 20 µg
    Specification HeLa Cells
    Treatment SiRNA Luciferase 10nM, 20nM, 24 hours
    Gel Running Conditions Non-reduced Denaturing (10)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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    Submitted Nov 07 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"