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Anti-Activin antibody [MM0074-7L18] (ab89307)

Abreviews (2)Q&A (1)Specific References (1)

Overview

  • Product nameAnti-Activin antibody [MM0074-7L18]
  • Description
    Mouse monoclonal [MM0074-7L18] to Activin
  • SpecificityDetects both pro- and mature Activin A.
  • Tested applicationsWB, Neutralising more details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant full length Human Precursor Activin A

Properties

Applications

Our Abpromise guarantee covers the use of ab89307 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/500 - 1/1000. Predicted molecular weight: 56 kDa.
Neutralising Neut: Use at an assay dependent dilution. (1) Activin A can induce hemoglobin expression in the K562 human chronic myelogenous leukemia cell line. (2) Anti-Activin antibody can neutralize Activin A induced hemoglobin expression in a dose dependent manner. ED 50 is approximately between 30-60ng/mL in the presence of 5ng/mL Recombinant Human Activin A.

Target

  • FunctionInhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.
  • Sequence similaritiesBelongs to the TGF-beta family.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P08476 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        Activin A antibodyActivin AB alpha polypeptide antibodyActivin AB antibody
        Activin AB beta polypeptide antibodyActivin B antibodyActivin beta A chain antibodyActivin beta B chain antibodyActivin beta-A chain antibodyActivinA antibodyActivinAB antibodyActivinB antibodyEDF antibodyErythroid differentiation factor antibodyErythroid differentiation protein antibodyfollicle stimulating hormone releasing protein antibodyFRP antibodyFSH releasing factor antibodyFSH releasing protein antibodyINHBA antibodyINHBA_HUMAN antibodyINHBB antibodyInhibin antibodyInhibin beta 1 antibodyInhibin beta 2 antibodyInhibin beta A antibodyInhibin beta A chain antibodyInhibin beta A subunit antibodyInhibin beta B antibodyInhibin beta B chain antibodyInhibin beta B subunit antibodyInhibin, beta A (activin A, activin AB alpha polypeptide) antibody
      see all

    Anti-Activin antibody [MM0074-7L18] images

    • Western blot - Activin antibody [MM0074-7L18] (ab89307)
      Western blot - Activin antibody [MM0074-7L18] (ab89307)
      All lanes : Anti-Activin antibody [MM0074-7L18] (ab89307) at 1 µg/ml

      Lane 1 : Embryonic Day 18 Liver
      Lane 2 : Embryonic Day 18 Muscles
      Lane 3 : Embryonic Day 18 Cortex (Negative Control)

      developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 56 kDa


      Exposure time : 2 minutes

      Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom

      See Abreview

    References for Anti-Activin antibody [MM0074-7L18] (ab89307)

    This product has been referenced in:
    • Karve TM  et al. BRCA1 regulates follistatin function in ovarian cancer and human ovarian surface epithelial cells. PLoS One 7:e37697 (2012). WB ; Human . Read more (PubMed: 22685544) »

    See 1 Publication for this product

    Publishing research using ab89307 ? Please let us know so that we can cite the reference in this datasheet

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-Activin antibody [MM0074-7L18]

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    Abreviews
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (human glioblastoma cell line)
    Specification human glioblastoma cell line
    Fixative Paraformaldehyde
    Permeabilization Yes - Tritonx100 0.1% in PBS
    Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
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    Submitted Apr 29 2011

    Western blot abreview for Anti-Activin antibody [MM0074-7L18]

     Good
    Abreviews
    Application Western blot
    Sample Rat Tissue lysate - whole (liver, muscle, brain)
    Loading amount 20 µg
    Specification liver, muscle, brain
    Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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    Verified customer

    Submitted Sep 07 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"