Anti-160 kD Neurofilament Medium antibody - Neuronal Marker (ab9034)
Overview
- Product nameAnti-160 kD Neurofilament Medium antibody - Neuronal MarkerSee all 160 kD Neurofilament Medium primary antibodies ...
- DescriptionRabbit polyclonal to 160 kD Neurofilament Medium - Neuronal Marker
- SpecificityRecognizes the evolutionarily conserved extreme C-terminal region of the medium neurofilament subunit (~145-170 kDa).
- Tested applicationsIHC-FoFr, IHC-Fr, IHC-P, WB, ICC/IF more details
- Species reactivityReacts with: Rat, Cow, Human
Predicted to work with: Mouse, Cat, Pig, Bird, Reptiles - Immunogen
Recombinant C-terminal fragment (Rat).
- Positive controlRat spinal cord
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- PurityWhole antiserum
- Clonality Polyclonal
- IsotypeIgG
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Research Areas
Applications
Our Abpromise guarantee covers the use of ab9034 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| IHC-FoFr | |
| IHC-Fr | |
| IHC-P | |
| WB | |
| ICC/IF |
IHC-F: 1/1000.
IHC-P: 1/1000.
IHC-Fr: 1/1000.
WB: 1/5000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
- RelevanceNeurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called neurofilament light (NF-L), neurofilament medium (NF-M) and neurofilament heavy (NF-H). Neurofilament medium runs on SDS-PAGE gels in the range 145-170 kDa, with some variation in different species. Antibodies to this protein are useful to identify neurons and their processes in tissue sections and in tissue culture. Neurofilament medium can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.
- Cellular localizationCytoplasm
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Database links
- Entrez Gene: 281347 Cow
- Entrez Gene: 4741 Human
- Entrez Gene: 18040 Mouse
- Entrez Gene: 24588 Rat
- Omim: 162250 Human
- SwissProt: O77788 Cow
- SwissProt: P07197 Human
- SwissProt: P08553 Mouse
- SwissProt: P12839 Rat
- Unigene: 458657 Human
- Unigene: 10971 Rat
see all
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Alternative names
- 150kDa medium antibody160 kDa neurofilament protein antibodyNEF 3 antibody
- NEF3 antibodyNEFM antibodyNeurofilament 3 antibodyNeurofilament medium polypeptide antibodyNeurofilament protein medium antibodyNeurofilament triplet M protein antibodyNeurofilament3 antibodyNF M antibodyNF160 antibodyNFM antibody
see all
Anti-160 kD Neurofilament Medium antibody - Neuronal Marker images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 160 kD Neurofilament Medium antibody - Neuronal Marker (ab9034)The image illustrates the high definition dendritic staining obtained after protease pretreatment. This picture was kindly supplied as part of the review submitted by Prof Colm Cunningham and Dr Suzanne Campion (Southampton University). -
Western blot - 160 kD Neurofilament Medium antibody - Neuronal Marker (ab9034)Anti-160 kD Neurofilament Medium antibody - Neuronal Marker (ab9034) at 1/20000 dilution + whole rat cerebellum homogenate -
Immunocytochemistry/ Immunofluorescence - 160 kD Neurofilament Medium antibody - Neuronal Marker (ab9034)ICC/IF image of ab9034 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9034, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Western blotAll lanes : Anti-160 kD Neurofilament Medium antibody - Neuronal Marker (ab9034) at 1/5000 dilution
Lane 1 :160 kD Neurofilament Medium protein (ab73759) at 0.1 µg
Lane 2 :160 kD Neurofilament Medium protein (ab73759) at 0.01 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 1 minute
References for Anti-160 kD Neurofilament Medium antibody - Neuronal Marker (ab9034)
This product has been referenced in:
- Deng Y et al. Dysregulation of insulin signaling, glucose transporters, O-GlcNAcylation, and phosphorylation of tau and neurofilaments in the brain: Implication for Alzheimer's disease. Am J Pathol 175:2089-98 (2009). WB, IHC-P ; Rat . Read more (PubMed: 19815707) »
- Deng Y et al. Regulation between O-GlcNAcylation and phosphorylation of neurofilament-M and their dysregulation in Alzheimer disease. FASEB J 22:138-45 (2008). Read more (PubMed: 17687114) »
