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Overview

  • Product nameMAP1B protein (Tagged-His Tag)
  • Protein descriptionRecombinant fragment, corresponding to amino acids 1-191 of Human MAP1B with an N-terminal His tag; Predicted MWt 22 kDa (UniProt P46821).
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 155 µl aqua dest
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch)
  • Sequence notesMATVVVEATEPEPSGSIANPAASTSPSLSHRFLDSKFYLL VVVGEIVTEE HLRRAIGNIELGIRSWDTNLIECNLDQE LKLFVSRHSARFSPEVPGQKIL HHRSDVLETVVLINPS DEAVSTEVRLMITDAARHKLLVLTGQCFENTGEL ILQS GSFSFQNFIEIFTDQEIGELLSTTHPANKASLTLFCP
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91700 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    MS
    SDS-PAGE
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        FUTSCHLC1MAP5
        Microtubule associated protein 1BMtap1bMtap5
      see all
  • RelevanceMicrotubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein t (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5. MAP1B is the major microtubule associated protein in developing brain which changes its expression during development. In the newborn rat brain, it is a major component of microtubules but in the adult its level is ten fold lower. This change in the level of expression occurs simultaneously with neuronal maturation. MAP1B is the first MAP to appear in growing axons during development as it is present from the first emergence of the nascent axon from the cell body.
  • Cellular localizationCytoplasmic

    • MAP1B protein (Tagged-His Tag) images

    • SDS-PAGE - MAP1B protein (Tagged-His Tag) (ab91700)
      SDS-PAGE - MAP1B protein (Tagged-His Tag) (ab91700)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for MAP1B protein (Tagged-His Tag) (ab91700)

    ab91700 has not yet been referenced specifically in any publications.

    Publishing research using ab91700 ? Please let us know so that we can cite the reference in this datasheet

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