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Overview

  • Product namePSME1 protein (Tagged-His Tag)See all PSME1 proteins and peptides ...
  • Protein descriptionRecombinant full length protein, corresponding to amino acids 1-250 of Human PSME1 with N terminal His tag, 34kDa. UniProt ID = Q06323.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 110 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch).
  • Sequence notesMAMLRVQPEAQAKVDVFREDLCTKTENLLGSYFPKKISEL DAFLKEPALNEANL SNLKAPLDIPVPDPVKEKEKEERK KQQEKEDKDEKKKGEDEDKGPPCGPVNCNE KIVVLLQR LKPEIKDVIEQLNLVTTWLQLQIPRIEDGNNFGVAVQEKV FELMTS LHTKLEGFHTQISKYFSERGDAVTKAAKQPHV GDYRQLVHELDEAEYRDIRLMV MEIRNAYVRRQGQGRG GQRQLSQATHSLTLQARG
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91718 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    MS
    SDS-PAGE
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        11S regulator complex alpha subunit11S regulator complex subunit alpha29 kD MCP activator subunit
        29kD MCP activator subunitActivator of multicatalytic protease subunit 1IFI5111IGUP I-5111Interferon gamma IEF SSP 5111Interferon gamma inducible protein 5111Interferon gamma up regulated I 5111 proteinInterferon gamma up-regulated I-5111 proteinMGC8628PA28APA28alphaProteasome (prosome, macropain) activator subunit 1 (PA28 alpha)Proteasome activator 28 subunit alphaProteasome activator complex subunit 1Proteasome activator subunit 1PSME1PSME1_HUMANREG-alphaREGalpha
      see all
  • FunctionImplicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.
  • Sequence similaritiesBelongs to the PA28 family.

    • Target information above from: UniProt accession Q06323 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    PSME1 protein (Tagged-His Tag) images

    • SDS-PAGE - PSME1 protein (Tagged-His Tag) (ab91718)
      SDS-PAGE - PSME1 protein (Tagged-His Tag) (ab91718)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for PSME1 protein (Tagged-His Tag) (ab91718)

    ab91718 has not yet been referenced specifically in any publications.

    Publishing research using ab91718 ? Please let us know so that we can cite the reference in this datasheet

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