You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Overview

  • Product nameSTAT6 protein (Tagged-His Tag)See all STAT6 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 1-287 of Human STAT6 with N terminal His tag; 287 amino acids, 33kDa. UniProt ID = P42226.
  • Expression hostE. coli

    • Properties

  • Purification notes"Purity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation."
  • FormLyophilised:Reconstitute with 147 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium hydrogen phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notes"Protein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch).
  • Sequence notesMSLWGLVSKMPPEKVQRLYVDFPQHLRHLLGDWLESQPWE FLVGSDAFCCNLA SALLSDTVQHLQASVGEQGEGSTIL QHISTLESIYQRDPLKLVATFRQILQGE KKAVMEQFRH LPMPFHWKQEELKFKTGLRRLQHRVGEIHLLREALQKGAE AGQ VSLHSLIETPANGTGPSEALAMLLQETTGELEAAK ALVLKRIQIWKRQQQLAG NGAPFEESLAPLQERCESLV DIYSQLQQEVGAAGGELEPKTRASLTGRLDEVL RTLVT SCFLVEKQPPQVLKTQT
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91735 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    MS
    SDS-PAGE
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        D12S1644IL 4 STATIL-4 Stat
        IL4 STATInterleukin 4 InducedInterleukin 4 Induced Transcription Factor IL4 STATSignal Transducer And Activator Of Transcription 6Signal Transducer And Activator Of Transcription 6 Interleukin 4 InducedSignal Transducer And Activator Of Transcription 6 Nirs Variant 1Signal transducer and activator of transcription 6, interleukin 4 inducedSTAT 6STAT interleukin4 inducedSTAT, interleukin4 inducedStat6STAT6_HUMANSTAT6BSTAT6CTranscription factor IL 4 STAT
      see all
  • FunctionCarries out a dual function: signal transduction and activation of transcription. Involved in interleukin-4 signalling.
  • Sequence similaritiesBelongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • Post-translational
    modifications    Tyrosine phosphorylated following stimulation by IL-4 and IL-3.
  • Cellular localizationCytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.

    • Target information above from: UniProt accession P42226 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    STAT6 protein (Tagged-His Tag) images

    • SDS-PAGE - STAT6 protein (Tagged-His Tag) (ab91735)
      SDS-PAGE - STAT6 protein (Tagged-His Tag) (ab91735)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for STAT6 protein (Tagged-His Tag) (ab91735)

    ab91735 has not yet been referenced specifically in any publications.

    Publishing research using ab91735 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit a Question for ab91735

    There are currently no Questions for ab91735.
    Please use the link above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"