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Overview

  • Product nameORP150 protein (Tagged-His Tag)See all ORP150 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 424-999 (576 amino acids) of Human ORP150 with N terminal His tag; Predicted MWt 65 kDa (UniProt Q9Y4L1).
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 129 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Sequence notesAALSKAFKVKPFVVRDAVVYPILVEFTREVEEEPGIHSLK HNKRVLFS RMGPYPQRKVITFNRYSHDFNFHINYGDLG FLGPEDLRVFGSQNLTTV KLKGVGDSFKKYPDYESKGI KAHFNLDESGVLSLDRVESVFETLVEDS AEEESTLTKL GNTISSLFGGGTTPDAKENGTDTVQEEEESPAEGSKDE PGEQVELKEEAEAPVEDGSQPPPPEPKGDATPEGEKATEK ENGDKSEA QKPSEKAEAGPEGVAPAPEGEKKQKPARKR RMVEEIGVELVVLDLPDL PEDKLAQSVQKLQDLTLRDL EKQEREKAANSLEAFIFETQDKLYQPEY QEVSTEEQRE EISGKLSAASTWLEDEGVGATTVMLKEKLAELRKLCQG LFFRVEERKKWPERLSALDNLLNHSSMFLKGARLIPEMDQ IFTEVEMT TLEKVINETWAWKNATLAEQAKLPATEKPV LLSKDIEAKMMALDREVQ YLLNKAKFTKPRPRPKDKNG TRAEPPLNASASDQGEKVIPPAGQTEDA EPISEPEKVE TGSEPGDTEPLELGGPGAEPEQKEQSTGQKRPLKNDEL
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91782 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
  • Application notesSDS-PAGE: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        150 kDa oxygen regulated protein150 kDa oxygen-regulated protein170 kDa glucose regulated protein
        170 kDa glucose-regulated proteinDKFZp686N08236FLJ94899FLJ97572glucose regulated protein 170Grp 170GRP-170Grp170HSP 12AHSP12AHYOU1HYOU1_HUMANHypoxia up regulated 1hypoxia up-regulated 1 precursorHypoxia up-regulated protein 1Hypoxia upregulated 1Orp 150ORP-150Orp150oxygen regulated protein (150kD)
      see all
  • FunctionHas a pivotal role in cytoprotective cellular mechanisms triggered by oxygen deprivation. May play a role as a molecular chaperone and participate in protein folding.
  • Tissue specificityHighly expressed in tissues that contain well-developed endoplasmic reticulum and synthesize large amounts of secretory proteins. Highly expressed in liver and pancreas and lower expression in brain and kidney. Also expressed in macrophages within aortic atherosclerotic plaques, and in breast cancers.
  • Sequence similaritiesBelongs to the heat shock protein 70 family.
  • Cellular localizationEndoplasmic reticulum lumen.

    • Target information above from: UniProt accession Q9Y4L1 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    ORP150 protein (Tagged-His Tag) images

    • SDS-PAGE - ORP150 protein (Tagged-His Tag) (ab91782)
      SDS-PAGE - ORP150 protein (Tagged-His Tag) (ab91782)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for ORP150 protein (Tagged-His Tag) (ab91782)

    ab91782 has not yet been referenced specifically in any publications.

    Publishing research using ab91782 ? Please let us know so that we can cite the reference in this datasheet

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