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Overview

  • Product nameATG4B protein (Tagged-His Tag)See all ATG4B proteins and peptides ...
  • Protein descriptionRecombinant full length protein, corresponding to amino acids 1-393 of Human ATG4B with N terminal His tag, 58kDa (44.3kDa predicted from sequence). UniProt ID = Q9Y4P1.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 65 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch).
  • Sequence notesMDAATLTYDTLRFAEFEDFPETSEPVWILGRKYSIFTEKD EILSDVASRLWFTYRKNF PAIGGTGPTSDTGWGCMLRC GQMIFAQALVCRHLGRDWRWTQRKRQPDSYFSVLNAFI DRKDSYYSIHQIAQMGVGEGKSIGQWYGPNTVAQVLKKLA VFDTWSSLAVHIAMDNTV VMEEIRRLCRTSVPCAGATA FPADSDRHCNGFPAGAEVTNRPSPWRPLVLLIPLRLGL TDINEAYVETLKHCFMMPQSLGVIGGKPNSAHYFIGYVGE ELIYLDPHTTQPAVEPTD GCFIPDESFHCQHPPCRMSI AELDPSIAVGFFCKTEDDFNDWCQQVKKLSLLGGALPM FELVELQPSHLACPDVLNLSLDSSDVERLERFFDSEDEDF EILSL
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91792 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    MS
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        APG4 autophagy 4 homolog BAPG4BATG4 autophagy related 4 homolog B (S. cerevisiae)
        ATG4BATG4B_HUMANAUT like 1 cysteine endopeptidaseAUT-like 1 cysteine endopeptidaseAUTL1Autophagin 1Autophagin-1Autophagy related cysteine endopeptidase 1Autophagy related protein 4 homolog BAutophagy-related cysteine endopeptidase 1Autophagy-related protein 4 homolog BCysteine protease ATG4BhAPG4BMGC1353
      see all
  • FunctionCysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.
  • Tissue specificityMainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas.
  • Sequence similaritiesBelongs to the peptidase C54 family.
  • Cellular localizationCytoplasm.

    • Target information above from: UniProt accession Q9Y4P1 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    ATG4B protein (Tagged-His Tag) images

    • SDS-PAGE - ATG4B protein (Tagged-His Tag) (ab91792)
      SDS-PAGE - ATG4B protein (Tagged-His Tag) (ab91792)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for ATG4B protein (Tagged-His Tag) (ab91792)

    ab91792 has not yet been referenced specifically in any publications.

    Publishing research using ab91792 ? Please let us know so that we can cite the reference in this datasheet

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