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Overview

  • Product nameFAM20C protein (Tagged-His Tag)
  • Protein descriptionRecombinant fragment, corresponding to amino acids 509-570 (62 aa) of Human FAM20C with a N terminal His tag; predicted MWt 17kDa (Uniprot ID: Q8IXL6).
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 112 µl distilled water.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch)
  • Sequence notesLLMAESLRGDQVAPVLYQPHLEALDRRLRVVLKAVRDCVE RDGLHSVVDDDLD TEHRAASAR
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91829 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    MS
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        C76981Dentin matrix protein 4DKFZp547C074
        DMP-4DMP4DMP4_HUMANExtracellular serine/threonine protein kinase Fam20CFam20cFamily with sequence similarity 20 member CGEF CKGolgi enriched fraction casein kinaseProtein FAM20CRNS
      see all
  • FunctionCalcium-binding protein which may play a role in dentin mineralization.
  • Tissue specificityWidely expressed.
  • Involvement in diseaseDefects in FAM20C are the cause of Raine syndrome (RNS) [MIM:259775]. RNS is an autosomal recessive osteosclerotic bone dysplasia with neonatal lethal outcome. Clinical features include generalized osteosclerosis, craniofacial dysplasia and microcephaly.
  • Sequence similaritiesBelongs to the FAM20 family.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession Q8IXL6 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    FAM20C protein (Tagged-His Tag) images

    • SDS-PAGE - FAM20C protein (Tagged-His Tag) (ab91829)
      SDS-PAGE - FAM20C protein (Tagged-His Tag) (ab91829)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images colored control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for FAM20C protein (Tagged-His Tag) (ab91829)

    ab91829 has not yet been referenced specifically in any publications.

    Publishing research using ab91829 ? Please let us know so that we can cite the reference in this datasheet

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"