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Overview

  • Product nameHAGH protein (Tagged-His Tag)See all HAGH proteins and peptides ...
  • Protein descriptionRecombinant full length protein, corresponding to amino acids 1-260 of Human HAGH isoform 2 with N terminal His tag, 37kDa (28.9kDa predicted from sequence). UniProt ID = Q16775.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 113 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch).
  • Sequence notesMKVEVLPALTDNYMYLVIDDETKEAAIVDPVQPQKVVDAA RKHGVKLTTVLTTHHHWDHA GGNEKLVKLESGLKVYGG DDRIGALTHKITHLSTLQVGSLNVKCLATPCHTSGHICYF VS KPGGSEPPAVFTGDTLFVAGCGKFYEGTADEMCKAL LEVLGRLPPDTRVYCGHEYTINNL KFARHVEPGNAAIR EKLAWAKEKYSIGEPTVPSTLAEEFTYNPFMRVREKTVQQ HAGETD PVTTMRAVRREKDQFKMPRD
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91866 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    MS
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        GLO2GLX IIGlyoxalase II
        HAGHHAGH1Hydroxyacyl glutathione hydrolaseHydroxyacylglutathione hydrolase, mitochondrial
      see all
  • RelevanceHydroxyacylglutathione hydrolase (HAGH) is a thiolesterase which hydrolyses S-lactoyl-glutathione to reduced glutathione and D-lactate.
  • Cellular localizationCytoplasmic and Mitochondrial

    • HAGH protein (Tagged-His Tag) images

    • SDS-PAGE - HAGH protein (Tagged-His Tag) (ab91866)
      SDS-PAGE - HAGH protein (Tagged-His Tag) (ab91866)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for HAGH protein (Tagged-His Tag) (ab91866)

    ab91866 has not yet been referenced specifically in any publications.

    Publishing research using ab91866 ? Please let us know so that we can cite the reference in this datasheet

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