Overview
Product namebeta COP protein (Tagged-His Tag)See all beta COP proteins and peptides ...
Protein descriptionRecombinant fragment, corresponding to amino acids 607-953 (347 amino acids) of Human beta COP with N terminal His tag; MWt 43 kDa (UniProt P53618).
Expression hostE. coli
Properties
Purification notesPurity level >85%
This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography.
In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag.
In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight.
This may be as a result of unequally distributed charges in the amino acid sequence.
Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
FormLyophilised:Reconstitute with 130 µl aqua dest.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Storage bufferPreservative: None
Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
Concentration information loading...
Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch)
Sequence notesDDDVDRISLCLKVLSECSPLMNDIFNKECRQSLSHMLSAK LEEEKLSQK
KESEKRNVTVQPDDPISFMQLTAKNEMNC KEDQFQLSLLAAMGNTQRKE
AADPLASKLNKVTQLTGF SDPVYAEAYVHVNQYDIVLDVLVVNQTSDTL
QNCTLEL ATLGDLKLVEKPSPLTLAPHDFANIKANVKVASTENGIIF GN
IVYDVSGAASDRNCVVLSDIHIDIMDYIQPATCTDA EFRQMWAEFEWEN
KVTVNTNMVDLNDYLQHILKSTNMK CLTPEKALSGYCGFMAANLYARSI
FGEDALANVSIEKP IHQGPDAAVTGHIRIRAKSQGMALSLGDKINLSQK
KTS I
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab91906
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
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Notes |
| SDS-PAGE |
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| MS |
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Application notesMS: Use at an assay dependent dilution.
SDS-PAGE: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
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Alternative names
Beta-coat proteinbetacopCoatomer beta subunit
Coatomer protein complex subunit betaCoatomer protein complex subunit beta 1Coatomer subunit betaCOPBCOPB1DKFZp761K102FLJ10341
see all
RelevanceThe Golgi complex is a key organelle where processing and sorting of newly synthesized proteins occurs. Membrane traffic from the endoplasmic reticulum (ER) to the Golgi complex and from the Golgi complex to the different final cellular destinations is believed to be mediated by carrier vesicles. Two populations of coated vesicles mediate biosynthetic membrane traffic between the different membrane-bound compartments. Clathrin-coated vesicles carry proteins to endocytic organelles and secretory granules, whilst non-clathrin-coated vesicles are involved in intra-Golgi transport and transport from the ER to the Golgi complex. Beta COP is a member of a set of protein which are believed to associate with the non-clathrin coated vesicles. Golgi-derived non-clathrin-coated vesicles are believed to act as bulk carriers, whereas clathrin-coated vesicles carry a selective cargo of membrane proteins.
Cellular localizationThe coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it.
beta COP protein (Tagged-His Tag) images
References for beta COP protein (Tagged-His Tag) (ab91906)
ab91906
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
SDS-PAGE - beta COP protein (Tagged-His Tag) (ab91906)The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.