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Overview

  • Product namePFKFB4 protein (Tagged-His Tag)See all PFKFB4 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 150-469 (320 amino acids) of Human PFKFB4 with an N terminal His tag. Predicted MWt: 26 kDa; UniProt ID: Q16877.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 129 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch)
  • Sequence notesYKTFFVESICVDPEVIAANIVQVKLGSPDYVNRDSDEATE DFMRRIECYENSY ESLDEDLDRDLSYIKIMDVGQSYVV NRVADHIQSRIVYYLMNIHVTPRSIYLC RHGESELNLK GRIGGDPGLSPRGREFAKSLAQFISDQNIKDLKVWTSQMK RTI QTAEALGVPYEQWKVLNEIDAGVCEEMTYEEIQDN YPLEFALRDQDKYRYRYP KGESYEDLVQRLEPVIMELE RQENVLVICHQAVMRCLLAYFLDKAAEQLPYLK CPLHT VLKLTPVAYGCKVESIFLNVAAVNTHRDRPQNVDISRPPE EALVTVPA HQ
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91941 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    MS
    SDS-PAGE
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        6-bisphosphatase6-P2ase 46-P2ASE testis-type isozyme
        6-phosphofructo-2-kinase6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 46PF 2 K/Fru 2,6 P2ase 46PF 2 K/Fru 2,6 P2ase testis type isozyme6PF-2-K/Fru-26PF-2-K/Fru-2,6-P2aseBifunctional enzyme with kinase and biphosphatase activitiesF264_HUMANFructose-2Fructose-2,6-bisphosphatasePFK/FBPase 4PFKFB4
      see all
  • FunctionSynthesis and degradation of fructose 2,6-bisphosphate.
  • Sequence similaritiesIn the C-terminal section; belongs to the phosphoglycerate mutase family.

    • Target information above from: UniProt accession Q16877 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    PFKFB4 protein (Tagged-His Tag) images

    • SDS-PAGE - PFKFB4 protein (Tagged-His Tag) (ab91941)
      SDS-PAGE - PFKFB4 protein (Tagged-His Tag) (ab91941)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for PFKFB4 protein (Tagged-His Tag) (ab91941)

    ab91941 has not yet been referenced specifically in any publications.

    Publishing research using ab91941 ? Please let us know so that we can cite the reference in this datasheet

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