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Overview

  • Product nameXRCC4 protein (Tagged-His Tag)
  • Protein descriptionRecombinant full length protein, corresponding to amino acids 1-334 of Human XRCC4 (Isoform 2) with N terminal His tag, Predicted MWt 39 kDa (UniProt Q13426-2).
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 91 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch).
  • Sequence notesMERKISRIHLVSEPSITHFLQVSWEKTLESGFVITLTDGH SAWTGTVSE SEISQEADDMAMEKGKYVGELRKALLSGA GPADVYTFNFSKESCYFFFE KNLKDVSFRLGSFNLEKV ENPAEVIRELICYCLDTIAENQAKNEHLQKE NERLLRD WNDVQGRFEKCVSAKEALETDLYKRFILVLNEKKTKIRSL HN KLLNAAQEREKDIKQEGETAICSEMTADRDPVYDES TDEESENQTDLSG LASAAVSKDDSIISSLDVTDIAPSR KRRQRMQRNLGTEPKMAPQENQLQ EKEKPDSSLPETSK KEHISAENMSLETLRNSSPEDLFDEI
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91973 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    MS
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        DNA double strand break repair and V(D)J recombination protein XRCC4DNA repair protein XRCC4X ray repair complementing defective repair in Chinese hamster cells 4
        X ray repair cross complementing protein 4X-ray repair cross-complementing protein 4XRCC 4XRCC4XRCC4_HUMAN
      see all
  • FunctionInvolved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesBelongs to the XRCC4 family.
  • Post-translational
    modifications    Phosphorylated by PRKDC. The phosphorylation seems not to be necessary for binding to DNA. Phosphorylation by CK2 promotes interaction with APTX.
    Monoubiquitinated.
    Sumoylation at Lys-210 is required for nuclear localization and recombination efficiency. Has no effect on ubiquitination.
  • Cellular localizationNucleus.

    • Target information above from: UniProt accession Q13426 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    XRCC4 protein (Tagged-His Tag) images

    • SDS-PAGE - XRCC4 protein (Tagged-His Tag) (ab91973)
      SDS-PAGE - XRCC4 protein (Tagged-His Tag) (ab91973)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for XRCC4 protein (Tagged-His Tag) (ab91973)

    ab91973 has not yet been referenced specifically in any publications.

    Publishing research using ab91973 ? Please let us know so that we can cite the reference in this datasheet

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