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Overview

  • Product nameHNRNPA0 protein (Tagged-His Tag)See all HNRNPA0 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 122-305 of Human HNRNPA0 with N terminal His tag; 184 amino acids, 30kDa (17.9kDa predicted from sequence). UniProt ID = Q13151.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 62 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Sequence notesGTVEKAEIIADKQSGKKRGFGFVYFQNHDAADKAAVVKFH PIQGHRVEVKKAVPKEDIYSG GGGGGSRSSRGGRGGRG RGGGRDQNGLSKGGGGGYNSYGGYGGGGGGGYNAYGGGGG GSSY GGSDYGNGFGGFGSYSQHQSSYGPMKSGGGGGGG GSSWGGRSNSGPYRGGYGGGGGYGGSSF
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab92006 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
  • Application notesSDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        Heterogeneous nuclear ribonucleoprotein A0hnRNA binding proteinhnRNP A0
        hnRNPA0ROA0_HUMAN
      see all
  • FunctionThis protein is a component of ribonucleosomes.
  • Sequence similaritiesContains 2 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications    Arg-291 is dimethylated, probably to asymmetric dimethylarginine.
  • Cellular localizationNucleus. Component of ribonucleosomes.

    • Target information above from: UniProt accession Q13151 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    HNRNPA0 protein (Tagged-His Tag) images

    • SDS-PAGE - HNRNPA0 protein (Tagged-His Tag) (ab92006)
      SDS-PAGE - HNRNPA0 protein (Tagged-His Tag) (ab92006)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for HNRNPA0 protein (Tagged-His Tag) (ab92006)

    ab92006 has not yet been referenced specifically in any publications.

    Publishing research using ab92006 ? Please let us know so that we can cite the reference in this datasheet

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