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Enah/Vasp-like protein (Tagged-His Tag) (ab92172)

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Overview

  • Product nameEnah/Vasp-like protein (Tagged-His Tag)
  • Protein descriptionRecombinant fragment, corresponding to amino acids 26-268 of Human Enah/Vasp-like with N terminal His tag; 243 amino acids, 28kDa (25.4kDa predicted from sequence). UniProt ID = Q9UI08.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 99 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch).
  • Sequence notesVPIKPGQQGFSRINIYHNTASNTFRVVGVKLQDQQVVINY SIVKGLKYNQATPTFHQWR DARQVYGLNFASKEEATTF SNAMLFALNIMNSQEGGPSSQRQVQNGPSPDEMDIQRRQV MEQHQQQRQESLERRTSATGPILPPGHPSSAASAPVSC SGPPPPPPPPVPPPPTGATPP PPPPLPAGGAQGSSHDE SSMSGLAAAIAGAKLRRVQRPEDASGGSSPSGTSKSDANR AS SGGGGGG
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab92172 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    MS
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        AI528774Ena-vasodilator stimulated phosphoproteinEna/vasodilator stimulated phosphoprotein like
        Ena/VASP like proteinEVLRNB 6RNB6
      see all
  • RelevanceEnah/Vasp-like (EVL) is a member of the Ena/VASP protein family. Ena/VASP proteins are actin-associated proteins involved in a range of processes that require dynamic actin remodeling such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. EVL enhances actin nucleation and polymerization. There are two different isoforms, generated by alternative splicing.
  • Cellular localizationCytoplasm, cytoskeleton. Lamellipodium.

    • Enah/Vasp-like protein (Tagged-His Tag) images

    • SDS-PAGE - Enah/Vasp-like protein (Tagged-His Tag) (ab92172)
      SDS-PAGE - Enah/Vasp-like protein (Tagged-His Tag) (ab92172)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for Enah/Vasp-like protein (Tagged-His Tag) (ab92172)

    ab92172 has not yet been referenced specifically in any publications.

    Publishing research using ab92172 ? Please let us know so that we can cite the reference in this datasheet

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