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Overview

  • Product nameFBXO31 protein (Tagged-His Tag)See all FBXO31 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 298-539 (242 amino acids) of Human FBXO31 with an N terminal His tag. Predicted MWt: 28 kDa (UniProt Q5XUX0).
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 138 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch
  • Sequence notesDLIKPGLFKGTYGSHGLEIVMLSFHGRRARGTKITGDPNI PAGQQTVEI DLRHRIQLPDLENQRNFNELSRIVLEVRE RVRQEQQEGGHEAGEGRGRQ GPRESQPSPAQPRAEAPS KGPDGTPGEDGGEPGDAVAAAEQPAQCGQGQ PFVLPVG VSSRNEDYPRTCRMCFYGTGLIAGHGFTSPERTPGVFILF DE DRFGFVWLELKSFSLYSRVQATFRNADAPSPQAFDE MLKNIQSLTS
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab92209 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    MS
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        DKFZp434B027DKFZp434J1815F box only protein 31
        F box protein 31F-box only protein 31FBX14FBX31FBX31_HUMANFBXO14Fbxo31FLJ22477MGC15419MGC9527pp2386Putative breast cancer tumor suppressorSCF ubiquitin ligase specificity factor
      see all
  • FunctionComponent of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in G1 arrest following DNA damage. Specifically recognizes phosphorylated cyclin-D1 (CCND1), promoting its ubiquitination and degradation by the proteasome, resulting in G1 arrest. May act as a tumor suppressor.
  • Tissue specificityHighly expressed in brain. Expressed at moderate levels in most tissues, except bone marrow.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the FBXO31 family.
    Contains 1 F-box domain.
  • Developmental stageExpression is cell-cycle regulated, and peaks at late G2 to early G1 phase (at protein level).
  • Post-translational
    modifications    Phosphorylation at Ser-278 by ATM following gamma-irradiation results in its stabilization.

    • Target information above from: UniProt accession Q5XUX0 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    FBXO31 protein (Tagged-His Tag) images

    • SDS-PAGE - FBXO31 protein (Tagged-His Tag) (ab92209)
      SDS-PAGE - FBXO31 protein (Tagged-His Tag) (ab92209)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for FBXO31 protein (Tagged-His Tag) (ab92209)

    ab92209 has not yet been referenced specifically in any publications.

    Publishing research using ab92209 ? Please let us know so that we can cite the reference in this datasheet

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