Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)

Overview

• Product nameAnti-Cytokeratin 13 antibody [EPR3671]See all Cytokeratin 13 primary antibodies ...
• Description
  Rabbit monoclonal [EPR3671] to Cytokeratin 13
• Tested applicationsIHC-Fr, WB, ICC, IHC-P more details
• Species reactivity
  Reacts with: Mouse, Human
  

  Does not react with

  Rat
• Immunogen

  Synthetic peptide (Human)

• Positive controlWB: A431, HACAT or 293T cell lysate. IHC: transitional cell urinary bladder carcinoma tissue.
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Dissociation constant (Kd) Kd = 1.20 x 10^-11 M
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEPR3671
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Intermediate Filaments
  • Class I
  • Cytokeratins

  • Tags & Cell Markers
  • Cell Type Markers
  • Epi / Endo-thelial

Applications

Target

• Tissue specificityExpressed in some epidermal sweat gland ducts (at protein level) and in exocervix, esophagus and placenta.
• Involvement in diseaseDefects in KRT13 are a cause of white sponge nevus of cannon (WSN) [MIM:193900]. WSN is a rare autosomal dominant disorder which predominantly affects non-cornified stratified squamous epithelia. Clinically, it is characterized by the presence of soft, white, and spongy plaques in the oral mucosa. The characteristic histopathologic features are epithelial thickening, parakeratosis, and vacuolization of the suprabasal layer of oral epithelial keratinocytes. Less frequently the mucous membranes of the nose, esophagus, genitalia and rectum are involved.
• Sequence similaritiesBelongs to the intermediate filament family.
• Post-translational
  modificationsO-glycosylated; glycans consist of single N-acetylglucosamine residues.

Target information above from: UniProt accession P13646 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 3860 Human
  • Entrez Gene: 16663 Mouse
  • Omim: 148065 Human
  • SwissProt: P13646 Human
  • SwissProt: P08730 Mouse
  • Unigene: 654550 Human
  • Unigene: 4646 Mouse

• Alternative names
  47 kDa cytokeratin antibodyCK-13 antibodyCK13 antibody

  Cytokeratin 13 antibodyCytokeratin-13 antibodyK13 antibodyK1C13_HUMAN antibodyKa13 antibodyKeratin 13 antibodyKeratin antibodykeratin type I cytoskeletal 13 antibodyKeratin-13 antibodyKrt-1.13 antibodyKrt1-13 antibodyKRT13 antibodyMGC161462 antibodyMGC3781 antibodytype I cytoskeletal 13 antibodyType I keratin Ka13 antibody

  see all

Anti-Cytokeratin 13 antibody [EPR3671] images

• 

  Western blot - Cytokeratin 13 antibody [EPR3671] (ab92551)
  All lanes : Anti-Cytokeratin 13 antibody [EPR3671] (ab92551) at 1/100000 dilution
  
  Lane 1 : A431 cell lysate
  Lane 2 : HACAT cell lysate
  Lane 3 : 293T cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  HRP labelled goat anti-Rabbit IgG at 1/2000 dilution
  
  Predicted band size : 50 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cytokeratin 13 antibody [EPR3671] (ab92551)
  ab92551 at 1/100 dilution staining Cytokeratin 13 in formalin-fixed, paraffin-embedded transitional cell usrinary bladder carcinoma tissue by immunohistochemistry. Detection: DAB staining.
• 

  Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 13 antibody [EPR3671] (ab92551)This image is courtesy of an anonymous Abreview
  ab92551 staining Cytokeratin 13 in Human vaginal epithelium tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.2% Triton in PBS and blocked with 1% fish skin gelatin for 30 minutes at 25°C. Samples were incubated with primary antibody (1/200) for 1 hour at 25°C. An Alexa Fluor^®594-conjugated Goat anti-rabbit polyclonal (1/1000) was used as the secondary antibody.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cytokeratin 13 antibody [EPR3671](ab92551)
  ab92551 showing positive staining in Cervical carcinoma tissue.
  
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cytokeratin 13 antibody [EPR3671](ab92551)
  ab92551 showing positive staining in Normal tonsil squamous cells tissue.
  
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cytokeratin 13 antibody [EPR3671](ab92551)
  ab92551 showing negative staining in Glioma tissue.
  
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cytokeratin 13 antibody [EPR3671](ab92551)
  ab92551 showing negative staining in Breast carcinoma tissue.
  
  
• 

  Other-Anti-Cytokeratin 13 antibody [EPR3671](ab92551)
  Equilibrium disassociation constant (K_D)
  Learn more about K_D
  
  Click here to learn more about K_D