Anti-SET antibody (ab92872)

Overview

• Product nameAnti-SET antibodySee all SET primary antibodies ...
• Description
  Rabbit polyclonal to SET
• Tested applicationsWB, IP more details
• Species reactivity
  Reacts with: Chicken, Human
  
• Immunogen

  Synthetic peptide, corresponding to a region within amino acids 125-175 (VTEFEDIKSG YRIDFYFDEN PYFENKVLSK EFHLNESGDP SSKSTEIKWK S) of Human SET, (NP_001116293.1).

• Positive controlHeLa and 293T cell lysate

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: 0.1% BSA, Tris buffered saline
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Oncoproteins/suppressors
  • Oncoproteins
  • Other

  • Signal Transduction
  • Protein Trafficking
  • ER Proteins

  • Epigenetics and Nuclear Signaling
  • Chromosome Structure
  • Chromatin assembly

  • Epigenetics and Nuclear Signaling
  • Chromatin Remodeling
  • Histone chaperones

  • Immunology
  • Adaptive Immunity
  • T Cells
  • Cytotoxic Cells

  • Cell Biology
  • Cell Cycle
  • Cell Division
  • Other cell division antibodies

Applications

Target

• FunctionMultitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone binding. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher.
• Tissue specificityWidely expressed. Low levels in quiescent cells during serum starvation, contact inhibition or differentiation. Highly expressed in Wilms' tumor.
• Involvement in diseaseNote=A chromosomal aberration involving SET is found in some cases of acute undifferentiated leukemia (AUL). Translocation t(6;9)(q21;q34.1) with NUP214/CAN.
• Sequence similaritiesBelongs to the nucleosome assembly protein (NAP) family.
• DomainThe C-terminal acidic domain mediates the inhibition of histone acetyltransferases and is required for the DNA replication stimulatory activity.
• Post-translational
  modificationsIsoform 2 is phosphorylated on Ser-15 and Thr-23.
  Isoform 2 is acetylated on Lys-11.
  Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.
  N-terminus of isoform 1 is methylated by METTL11A/NTM1. Mainly trimethylated.
• Cellular localizationCytoplasm > cytosol. Endoplasmic reticulum. Nucleus > nucleoplasm. In the cytoplasm, found both in the cytosol and associated with the endoplasmic reticulum. Following CTL attack, moves rapidly to the nucleus, where it is found in the nucleoplasm, avoiding the nucleolus. Similar translocation to the nucleus is also observed for lymphocyte-activated killer cells after the addition of calcium. The SET complex is associated with the endoplasmic reticulum.

Target information above from: UniProt accession Q01105 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 417210 Chicken
  • Entrez Gene: 6418 Human
  • NCBI: 1263308 Human
  • Omim: 600960 Human
  • SwissProt: Q01105 Human
  • Unigene: 436687 Human
  • Unigene: 596814 Human

• Alternative names
  2PP2A antibodyHLA DR associated protein II antibodyHLA-DR-associated protein II antibody

  I 2PP2A antibodyI-2PP2A antibodyI2PP2A antibodyIGAAD antibodyInhibitor of granzyme A activated DNase antibodyInhibitor of granzyme A-activated DNase antibodyPHAPII antibodyPhosphatase 2A inhibitor I2PP2A antibodyProtein SET antibodySet antibodySET translocation antibodySET translocation (myeloid leukemia-associated) antibodySET_HUMAN antibodyTAF I antibodyTAF IBETA antibodyTAF-I antibodyTAFI antibodyTemplate activating factor I antibodyTemplate-activating factor I antibody

  see all

Anti-SET antibody images

• 

  Western blot - SET antibody (ab92872)
  All lanes : Anti-SET antibody (ab92872) at 0.04 µg/ml
  
  Lane 1 : HeLa cell lysate at 50 µg
  Lane 2 : HeLa cell lysate at 15 µg
  Lane 3 : HeLa cell lysate at 5 µg
  Lane 4 : 293T cell lysate at 50 µg
  
  developed using the ECL technique
  
  Predicted band size : 33 kDa
  
  
  Exposure time : 30 seconds
• 

  Immunoprecipitation - SET antibody (ab92872)
  3µg ab92872 were used to immunoprecipitate SET from 1mg HeLa whole cell lysate. 20% IP was loaded and probed with ab92872 at 1µg/ml.
• 

  Western blot - SET antibody (ab92872)
  Anti-SET antibody (ab92872) at 1/2000 dilution ((in 3% milk in PBST). Incubation was for 24 hours at 4°C.) + Lysate of the soluble fraction of HwLa or U20S cells at 30 µg
  
  Secondary
  An HRP polymer Goat anti-rabbit IgG polyclonal at 1/10000 dilution
  developed using the ECL technique
  
  Predicted band size : 33 kDa
  Observed band size : ~37,~40 kDa (why is the actual band size different from the predicted?)
  
  
  Exposure time : 1 minute

  This image is courtesy of an anonymous Abreview

  See Abreview