Anti-GFAP antibody - Astrocyte Marker, prediluted (ab929)
Overview
- Product nameAnti-GFAP antibody - Astrocyte Marker, predilutedSee all GFAP primary antibodies ...
- DescriptionRabbit polyclonal to GFAP - Astrocyte Marker, prediluted
- SpecificityIn the central nervous system, this antibody stains astrocytes and some groups of ependymal cells. However, in the peripheral nervous system, it stains Schwann cells, satellite cells and enteric glial cells.
- Tested applicationsICC/IF, IHC-P, IHC-Fr more details
- Species reactivityReacts with: Mouse, Rat, Sheep, Chicken, Guinea pig, Hamster, Cow, Cat, Human, Kangaroo, Monkey
- Immunogen
Glial fibrillary acidic protein isolated from cow spinal cord.
- General notesGFAP is Glial Fibrillary Acidic Protein.
Properties
- FormPrediluted
- Storage instructionsStore at +4°C. Do not freeze.
- Storage bufferPreservative: 0.05% Sodium Azide
Constituents: Inert stabilizer -
Concentration information loading... - PurityProtein A purified
- Clonality Polyclonal
- Myelomaunknown
- IsotypeIgG
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Research Areas
Applications
Our Abpromise guarantee covers the use of ab929 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC/IF | |
| IHC-P | |
| IHC-Fr |
IHC-P: Use at an assay dependent concentration.
IHC-Fr: Use at an assay dependent concentration.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
- FunctionGFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
- Tissue specificityExpressed in cells lacking fibronectin.
- Involvement in diseaseDefects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
- Sequence similaritiesBelongs to the intermediate filament family.
- Post-translational
modificationsPhosphorylated by PKN1. - Cellular localizationCytoplasm. Associated with intermediate filaments.
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Database links
- Entrez Gene: 281189 Cow
- Entrez Gene: 2670 Human
- Entrez Gene: 14580 Mouse
- Entrez Gene: 24387 Rat
- Omim: 137780 Human
- SwissProt: Q28115 Cow
- SwissProt: P14136 Human
- SwissProt: P03995 Mouse
- SwissProt: P47819 Rat
- Unigene: 514227 Human
- Unigene: 1239 Mouse
- Unigene: 91512 Rat
see all
Target information above from: UniProt accession
P14136
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Astrocyte antibodyFLJ42474 antibodyFLJ45472 antibody
- GFAP antibodyGFAP_HUMAN antibodyGlial Fibrillary Acidic Protein antibodyIntermediate filament protein antibody
see all
Anti-GFAP antibody - Astrocyte Marker, prediluted images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GFAP antibody - Astrocyte Marker, prediluted (ab929)Ab929 staining human substantia nigra. Staining is localised to the cytoplasm.
Left panel: with primary antibody at 1-4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
References for Anti-GFAP antibody - Astrocyte Marker, prediluted (ab929)
This product has been referenced in:
- Wang B et al. Nogo-66 promotes the differentiation of neural progenitors into astroglial lineage cells through mTOR-STAT3 pathway. PLoS ONE 3:e1856 (2008). ICC/IF ; Rat . Read more (PubMed: 18365011) »
- Zhang J et al. Olfactory ensheathing cells promote proliferation and inhibit neuronal differentiation of neural progenitor cells through activation of Notch signaling. Neuroscience 153:406-13 (2008). ICC/IF ; Rat . Read more (PubMed: 18400409) »
