Anti-Estrogen Related Receptor alpha antibody (ab93173)

Overview

• Product nameAnti-Estrogen Related Receptor alpha antibodySee all Estrogen Related Receptor alpha primary antibodies ...
• Description
  Rabbit polyclonal to Estrogen Related Receptor alpha
• Tested applicationsIHC-P, Flow Cyt, WB more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  KLH conjugated synthetic peptide selected from the Center region of Human Estrogen Related Receptor alpha (NP_004442.3).

• Positive controlWB: A375 cell line lysates IHC-P: human kidney carcinoma FC: 293 cells

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: PBS
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesab93173 is purified through a protein A column, followed by peptide affinity purification.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Mitochondrial Biogenesis

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Nucleotide metabolism
  • Molecular processes
  • Mitochondrial transcription

  • Cancer
  • Signal transduction
  • Nuclear signaling
  • Nuclear hormone receptors
  • Estrogen

  • Signal Transduction
  • Signaling Pathway
  • Nuclear Signaling
  • Nuclear Hormone Receptors
  • Estrogen

  • Epigenetics and Nuclear Signaling
  • Nuclear Signaling Pathways
  • Nuclear Receptors
  • Estrogen

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • Zinc Finger

Applications

Target

• FunctionBinds to an ERR-alpha response element (ERRE) containing a single consensus half-site, 5'-TNAAGGTCA-3'. Can bind to the medium-chain acyl coenzyme A dehydrogenase (MCAD) response element NRRE-1 and may act as an important regulator of MCAD promoter. Binds to the C1 region of the lactoferrin gene promoter. Requires dimerization and the coactivator, PGC-1A, for full activity. The ERRalpha/PGC1alpha complex is a regulator of energy metabolism.
• Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
  Contains 1 nuclear receptor DNA-binding domain.
• Post-translational
  modificationsPhosphorylation on Ser-19 enhances sumoylation on Lys-14 increasing repression of transcriptional activity.
  Sumoylated by SUMO2. Main site is Lys-14 which is enhanced by phosphorylation on Ser-19, cofactor activation, and by interaction with PIAS4. Sumoylation enhances repression of transcriptional activiy, but has no effect on subcellular location nor on DNA binding.
• Cellular localizationNucleus.

Target information above from: UniProt accession P11474 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 2101 Human
  • Omim: 601998 Human
  • SwissProt: P11474 Human
  • Unigene: 110849 Human

• Alternative names
  Err 1 antibodyERR a antibodyERR alpha antibody

  ERR-alpha antibodyErr1 antibodyERR1 protein antibodyERR1_HUMAN antibodyERRa antibodyERRalpha antibodyESRL 1 antibodyESRL1 antibodyESRR A antibodyEsrra antibodyEstrogen receptor like 1 antibodyEstrogen receptor related 1 antibodyestrogen receptor related receptor alpha antibodyEstrogen receptor-like 1 antibodyEstrogen related receptor alpha antibodyEstrogen-related receptor alpha antibodyEstrra antibodyhERR1 antibodyNR3B1 antibodyNuclear receptor subfamily 3 group B member 1 antibodySteroid hormone receptor ERR1 antibody

  see all

Anti-Estrogen Related Receptor alpha antibody images

• 

  Western blot - Estrogen Related Receptor alpha antibody (ab93173)
  Anti-Estrogen Related Receptor alpha antibody (ab93173) at 1/100 dilution + A375 cell line lysate at 35 µg
  
  Predicted band size : 55 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Estrogen Related Receptor alpha antibody (ab93173)
  ab93173, at a 1/50 dilution, staining Estrogen Related Receptor alpha in formalin-fixed and paraffin-embedded Human kidney carcinoma, peroxidase-conjugated to a FITC-conjugated goat-anti-rabbit secondary antibody, followed by DAB staining.
• 

  Flow Cytometry - Estrogen Related Receptor alpha antibody (ab93173)
  ab93173, at a 1/10 dilution, staining Estrogen Related Receptor alpha in 293 cells by flow cytometric analysis (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibody used for analysis.